Chlamydomonas reinhardtii Dangeard (Chlamydomonadales, Chlorophyceae) mutant with multiple eyespots

Shogo Nakamura, Haruo Ogihara, Kinue Jinbo, Midori Tateishi, Tetsuo Takahashi, Kenjiro Yoshimura, Mamoru Kubota, Masakatsu Watanabe, Soichi Nakamura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have isolated a new Chlamydomonas reinhardtii Dangeard (Chlamydomonadales, Chlorophyceae) mutant with from one up to more than four eyespots cell-1. It was designated mes (multiple eyespots)-10. A wild-type cell has a single eyespot, located under the chloroplast envelope, at a certain position near the cell's equator where the chloroplast envelope is in contact with the cell membrane. The eyespot(s) in mes-10, however, are located at various positions on its chloroplast. The mes-10 cells displayed negative phototaxis to 480-500 nm light. This behavior differed from that of a similar mutant, ptx4, which has been shown to have multiple eyespots and display no phototaxis (Pazour et al., J. Cell Biol. 1995; 131: 427-40). Mes-10 may retain a functional photoreceptor and a photosignal transduction system independently of its multiple eyespots. This mutant should be useful for studying how C. reinhardtii responds to light signals, as well as how eyespots are formed in the cell.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalPhycological Research
Volume49
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Chlorophyceae
Chlamydomonas reinhardtii
chloroplast
phototaxis
mutants
chloroplasts
cells
membrane
photoreceptors
cell membranes
Chlamydomonadales
mutant

Keywords

  • Chlamydomonas
  • Eyespot
  • Mutant
  • Photophobic response
  • Phototaxis

ASJC Scopus subject areas

  • Plant Science
  • Microbiology
  • Oceanography

Cite this

Chlamydomonas reinhardtii Dangeard (Chlamydomonadales, Chlorophyceae) mutant with multiple eyespots. / Nakamura, Shogo; Ogihara, Haruo; Jinbo, Kinue; Tateishi, Midori; Takahashi, Tetsuo; Yoshimura, Kenjiro; Kubota, Mamoru; Watanabe, Masakatsu; Nakamura, Soichi.

In: Phycological Research, Vol. 49, No. 2, 2001, p. 115-122.

Research output: Contribution to journalArticle

Nakamura, S, Ogihara, H, Jinbo, K, Tateishi, M, Takahashi, T, Yoshimura, K, Kubota, M, Watanabe, M & Nakamura, S 2001, 'Chlamydomonas reinhardtii Dangeard (Chlamydomonadales, Chlorophyceae) mutant with multiple eyespots', Phycological Research, vol. 49, no. 2, pp. 115-122. https://doi.org/10.1046/j.1440-1835.2001.00231.x
Nakamura, Shogo ; Ogihara, Haruo ; Jinbo, Kinue ; Tateishi, Midori ; Takahashi, Tetsuo ; Yoshimura, Kenjiro ; Kubota, Mamoru ; Watanabe, Masakatsu ; Nakamura, Soichi. / Chlamydomonas reinhardtii Dangeard (Chlamydomonadales, Chlorophyceae) mutant with multiple eyespots. In: Phycological Research. 2001 ; Vol. 49, No. 2. pp. 115-122.
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