Classification of halo(alkali)philic and halo(alkali)tolerant methanotrophs provisionally assigned to the genera Methylomicrobium and Methylobacter and emended description of the genus Methylomicrobium

Marina G. Kalyuzhnaya, Valentina Khmelenina, Bulat Eshinimaev, Dimitry Sorokin, Hiroyuki Fuse, Mary Lidstrom, Yuri Trotsenko

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The taxonomic positions of four aerobic, obligately halo(alkali)philic/ -tolerant, methanotrophic bacteria previously affiliated with the genera Methylobacter ('Methylobacter alcaliphilus' strains 20Z and 5Z) and Methylomicrobium (Methylomicrobium strains AMO1 and NI) were investigated. Phylogenetic analysis of 16S rRNA gene sequences indicated that the strains form a separate branch within the type I methanotrophic bacteria and are closely related to Methylomicrobium pelagicum. DNA-DNA hybridization data revealed relatively low levels of relatedness of Methylomicrobium sp. AMO1 and Methylomicrobium sp. N1 with each other and with previously described species of the genera Methylomicrobium and Methylobacter (<55 %), indicating that they may represent novel species. Based on the results presented here and on previously reported morphological and physiological characteristics, we classify these halotolerant and halophilic methanotrophic strains as representing novel species within the genus Methylomicrobium: Methylomicrobium alcaliphilum sp. nov. (type strain 20ZT =VKM B-2133T =NCIMB 14124T; reference strain 5Z =VKM B-2180), Methylomicrobium japanense sp. nov. (type strain NIT =VKM B-2462T =FERM BP-5633T =NBRC 103677T) and Methylomicrobium kenyense sp. nov. (type strain AMO1T =NCCB 97157T =NCIMB 13566T =VKM B-2464T). The genus Methylomicrobium has been emended in its description.

Original languageEnglish
Pages (from-to)591-596
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume58
Issue number3
DOIs
Publication statusPublished - 2008 Mar
Externally publishedYes

Fingerprint

Methylomicrobium
Methylobacter
methanotrophs
alkalis
taxonomy
Methylobacter alcaliphilus
DNA
bacterium
relatedness
Methylomicrobium pelagicum
phylogenetics
gene
new species
bacteria
nucleic acid hybridization

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Classification of halo(alkali)philic and halo(alkali)tolerant methanotrophs provisionally assigned to the genera Methylomicrobium and Methylobacter and emended description of the genus Methylomicrobium. / Kalyuzhnaya, Marina G.; Khmelenina, Valentina; Eshinimaev, Bulat; Sorokin, Dimitry; Fuse, Hiroyuki; Lidstrom, Mary; Trotsenko, Yuri.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 58, No. 3, 03.2008, p. 591-596.

Research output: Contribution to journalArticle

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abstract = "The taxonomic positions of four aerobic, obligately halo(alkali)philic/ -tolerant, methanotrophic bacteria previously affiliated with the genera Methylobacter ('Methylobacter alcaliphilus' strains 20Z and 5Z) and Methylomicrobium (Methylomicrobium strains AMO1 and NI) were investigated. Phylogenetic analysis of 16S rRNA gene sequences indicated that the strains form a separate branch within the type I methanotrophic bacteria and are closely related to Methylomicrobium pelagicum. DNA-DNA hybridization data revealed relatively low levels of relatedness of Methylomicrobium sp. AMO1 and Methylomicrobium sp. N1 with each other and with previously described species of the genera Methylomicrobium and Methylobacter (<55 {\%}), indicating that they may represent novel species. Based on the results presented here and on previously reported morphological and physiological characteristics, we classify these halotolerant and halophilic methanotrophic strains as representing novel species within the genus Methylomicrobium: Methylomicrobium alcaliphilum sp. nov. (type strain 20ZT =VKM B-2133T =NCIMB 14124T; reference strain 5Z =VKM B-2180), Methylomicrobium japanense sp. nov. (type strain NIT =VKM B-2462T =FERM BP-5633T =NBRC 103677T) and Methylomicrobium kenyense sp. nov. (type strain AMO1T =NCCB 97157T =NCIMB 13566T =VKM B-2464T). The genus Methylomicrobium has been emended in its description.",
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AU - Kalyuzhnaya, Marina G.

AU - Khmelenina, Valentina

AU - Eshinimaev, Bulat

AU - Sorokin, Dimitry

AU - Fuse, Hiroyuki

AU - Lidstrom, Mary

AU - Trotsenko, Yuri

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