Cloning and characterization of mouse mSox13 cDNA

Susumu Kido, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Yasunori Yoshimura, Sadakazu Aiso

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A novel SRY-related cDNA, mSox13, was isolated from a λ phage library derived from mouse embryo. The cDNA encodes a protein of 595 amino acids containing the SRY-type high mobility group (HMG) box and a putative leucine zipper motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named Q box, are well conserved among known type-D SOX proteins. The expression of mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay indicates that the recombinant mSox13 protein is capable of binding to the AACAAT sequence.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalGene
Volume208
Issue number2
DOIs
Publication statusPublished - 1998 Feb 22
Externally publishedYes

Keywords

  • DNA binding
  • Embryo
  • HMG box
  • Leucine zipper
  • SRY

ASJC Scopus subject areas

  • Genetics

Cite this

Kido, S., Hiraoka, Y., Ogawa, M., Sakai, Y., Yoshimura, Y., & Aiso, S. (1998). Cloning and characterization of mouse mSox13 cDNA. Gene, 208(2), 201-206. https://doi.org/10.1016/S0378-1119(97)00667-7

Cloning and characterization of mouse mSox13 cDNA. / Kido, Susumu; Hiraoka, Yoshiki; Ogawa, Motoyuki; Sakai, Yukinao; Yoshimura, Yasunori; Aiso, Sadakazu.

In: Gene, Vol. 208, No. 2, 22.02.1998, p. 201-206.

Research output: Contribution to journalArticle

Kido, S, Hiraoka, Y, Ogawa, M, Sakai, Y, Yoshimura, Y & Aiso, S 1998, 'Cloning and characterization of mouse mSox13 cDNA', Gene, vol. 208, no. 2, pp. 201-206. https://doi.org/10.1016/S0378-1119(97)00667-7
Kido S, Hiraoka Y, Ogawa M, Sakai Y, Yoshimura Y, Aiso S. Cloning and characterization of mouse mSox13 cDNA. Gene. 1998 Feb 22;208(2):201-206. https://doi.org/10.1016/S0378-1119(97)00667-7
Kido, Susumu ; Hiraoka, Yoshiki ; Ogawa, Motoyuki ; Sakai, Yukinao ; Yoshimura, Yasunori ; Aiso, Sadakazu. / Cloning and characterization of mouse mSox13 cDNA. In: Gene. 1998 ; Vol. 208, No. 2. pp. 201-206.
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