Cloning and characterization of the iutA gene which encodes ferric aerobactin receptor from marine Vibrio species

Katsuji Murakami, Hiroyuki Fuse, Osamu Takimura, Hiroyuki Inoue, Yukiho Yamaoka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The iutA gene from marine Vibrio species SD004, which encoded a ferric aerobactin receptor for the uptake of iron(III), was cloned onto a multicopy plasmid, pUC 18, in Escherichia coli. Identification of the positive clone was achieved on the basis of its deferrization activity and was detected as a halo formation on the chrome azurol S (CAS)-containing selective plate. Nucleotide sequence analysis of the cloned DNA fragment revealed an open reading frame (ORF) which encoded a polypeptide of 706 amino acid residues, and the deduced molecular mass of this polypeptide was 77.906 kD. The amino acid sequence showed a 41% homology with that of the IutA protein from E. coli. The cloned gene was iutA, which encoded the ferric aerobactin receptor. Another incomplete ORF was found 100 bp upstream of the iutA gene, which was homologous (31 out of 49 amino acids) with the C-terminal region of the lue D protein of E. coli. It is suggested that aerobactin biosynthesis and the transport genes are located tandemly on the Vibrio chromosome and may form an aerobactin operon.

Original languageEnglish
Pages (from-to)137-146
Number of pages10
JournalMicrobios
Volume101
Issue number400
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Aerobactin
  • IutA gene
  • Siderophore
  • Vibrio

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Cloning and characterization of the iutA gene which encodes ferric aerobactin receptor from marine Vibrio species. / Murakami, Katsuji; Fuse, Hiroyuki; Takimura, Osamu; Inoue, Hiroyuki; Yamaoka, Yukiho.

In: Microbios, Vol. 101, No. 400, 2000, p. 137-146.

Research output: Contribution to journalArticle

Murakami, K, Fuse, H, Takimura, O, Inoue, H & Yamaoka, Y 2000, 'Cloning and characterization of the iutA gene which encodes ferric aerobactin receptor from marine Vibrio species', Microbios, vol. 101, no. 400, pp. 137-146.
Murakami, Katsuji ; Fuse, Hiroyuki ; Takimura, Osamu ; Inoue, Hiroyuki ; Yamaoka, Yukiho. / Cloning and characterization of the iutA gene which encodes ferric aerobactin receptor from marine Vibrio species. In: Microbios. 2000 ; Vol. 101, No. 400. pp. 137-146.
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