Cloning and characterization of Xenopus laevis xSox7 cDNA

Masahide Shiozawa; Yoshiki Hiraoka; Naoki Komatsu; Motoyuki Ogawa; Yukinao Sakai; Sadakazu Aiso

doi:10.1016/S0167-4781(96)00145-5

Cloning and characterization of Xenopus laevis xSox7 cDNA

Masahide Shiozawa, Yoshiki Hiraoka, Naoki Komatsu, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

SIT Research Laboratories

Research output: Contribution to journal › Article

25 Citations (Scopus)

Abstract

A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

Original language	English
Pages (from-to)	73-76
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1309
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(96)00145-5
Publication status	Published - 1996 Nov 11

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Keywords

(Xenopus laevis)
Nucleotide sequence
SOX
SRY
cDNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Cite this

Shiozawa, M., Hiraoka, Y., Komatsu, N., Ogawa, M., Sakai, Y., & Aiso, S. (1996). Cloning and characterization of Xenopus laevis xSox7 cDNA. Biochimica et Biophysica Acta - Gene Structure and Expression, 1309(1-2), 73-76. https://doi.org/10.1016/S0167-4781(96)00145-5

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abstract = "A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.",

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AU - Sakai, Yukinao

AU - Aiso, Sadakazu

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10.1016/S0167-4781(96)00145-5