Cloning and characterization of Xenopus laevis xSox7 cDNA

Masahide Shiozawa, Yoshiki Hiraoka, Naoki Komatsu, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

Original languageEnglish
Pages (from-to)73-76
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1309
Issue number1-2
DOIs
Publication statusPublished - 1996 Nov 11
Externally publishedYes

Fingerprint

Cloning
Complementary DNA
Xenopus Proteins
Tissue
Electrophoretic mobility
Messenger RNA
Polymerase chain reaction
Transcription
Assays
Genes
Amino Acids

Keywords

  • (Xenopus laevis)
  • cDNA
  • Nucleotide sequence
  • SOX
  • SRY

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Cloning and characterization of Xenopus laevis xSox7 cDNA. / Shiozawa, Masahide; Hiraoka, Yoshiki; Komatsu, Naoki; Ogawa, Motoyuki; Sakai, Yukinao; Aiso, Sadakazu.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1309, No. 1-2, 11.11.1996, p. 73-76.

Research output: Contribution to journalArticle

Shiozawa, Masahide ; Hiraoka, Yoshiki ; Komatsu, Naoki ; Ogawa, Motoyuki ; Sakai, Yukinao ; Aiso, Sadakazu. / Cloning and characterization of Xenopus laevis xSox7 cDNA. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1996 ; Vol. 1309, No. 1-2. pp. 73-76.
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