Abstract
A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.
Original language | English |
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Pages (from-to) | 73-76 |
Number of pages | 4 |
Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
Volume | 1309 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 1996 Nov 11 |
Externally published | Yes |
Keywords
- (Xenopus laevis)
- Nucleotide sequence
- SOX
- SRY
- cDNA
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics