Cloning and characterization of Xenopus laevis xSox7 cDNA

Masahide Shiozawa; Yoshiki Hiraoka; Naoki Komatsu; Motoyuki Ogawa; Yukinao Sakai; Sadakazu Aiso

doi:10.1016/S0167-4781(96)00145-5

Cloning and characterization of Xenopus laevis xSox7 cDNA

Masahide Shiozawa, Yoshiki Hiraoka, Naoki Komatsu, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

Original language	English
Pages (from-to)	73-76
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1309
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(96)00145-5
Publication status	Published - 1996 Nov 11
Externally published	Yes

Keywords

(Xenopus laevis)
Nucleotide sequence
SOX
SRY
cDNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(96)00145-5

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title = "Cloning and characterization of Xenopus laevis xSox7 cDNA",

abstract = "A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.",

keywords = "(Xenopus laevis), Nucleotide sequence, SOX, SRY, cDNA",

author = "Masahide Shiozawa and Yoshiki Hiraoka and Naoki Komatsu and Motoyuki Ogawa and Yukinao Sakai and Sadakazu Aiso",

note = "Funding Information: We thank Dr. Chang Sim for reading the manuscript. This work was supported in part by a grant from the Ministry of Education, Science and Culture to S.A. and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research from Keio University to Y.H.",

year = "1996",

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doi = "10.1016/S0167-4781(96)00145-5",

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volume = "1309",

pages = "73--76",

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T1 - Cloning and characterization of Xenopus laevis xSox7 cDNA

AU - Shiozawa, Masahide

AU - Hiraoka, Yoshiki

AU - Komatsu, Naoki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Aiso, Sadakazu

N1 - Funding Information: We thank Dr. Chang Sim for reading the manuscript. This work was supported in part by a grant from the Ministry of Education, Science and Culture to S.A. and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research from Keio University to Y.H.

PY - 1996/11/11

Y1 - 1996/11/11

N2 - A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

AB - A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

KW - (Xenopus laevis)

KW - Nucleotide sequence

KW - SOX

KW - SRY

KW - cDNA

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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SN - 0167-4781

IS - 1-2

ER -

Cloning and characterization of Xenopus laevis xSox7 cDNA

Abstract

Keywords

ASJC Scopus subject areas

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