Abstract
A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.
| Original language | English |
|---|---|
| Pages (from-to) | 73-76 |
| Number of pages | 4 |
| Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Volume | 1309 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1996 Nov 11 |
| Externally published | Yes |
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Keywords
- (Xenopus laevis)
- cDNA
- Nucleotide sequence
- SOX
- SRY
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics
Cite this
Cloning and characterization of Xenopus laevis xSox7 cDNA. / Shiozawa, Masahide; Hiraoka, Yoshiki; Komatsu, Naoki; Ogawa, Motoyuki; Sakai, Yukinao; Aiso, Sadakazu.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1309, No. 1-2, 11.11.1996, p. 73-76.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and characterization of Xenopus laevis xSox7 cDNA
AU - Shiozawa, Masahide
AU - Hiraoka, Yoshiki
AU - Komatsu, Naoki
AU - Ogawa, Motoyuki
AU - Sakai, Yukinao
AU - Aiso, Sadakazu
PY - 1996/11/11
Y1 - 1996/11/11
N2 - A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.
AB - A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.
KW - (Xenopus laevis)
KW - cDNA
KW - Nucleotide sequence
KW - SOX
KW - SRY
UR - http://www.scopus.com/inward/record.url?scp=0030580370&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030580370&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(96)00145-5
DO - 10.1016/S0167-4781(96)00145-5
M3 - Article
C2 - 8950180
AN - SCOPUS:0030580370
VL - 1309
SP - 73
EP - 76
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1-2
ER -