Cloning, Sequencing and Characterisation of a Downstream Region of ksdDI Operon of Arthrobacter simplex

Jarosław Dziadek, Mitsuo Yamashita, Yoshikatsu Murooka

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An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90% identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalActa Microbiologica Polonica
Issue number4
Publication statusPublished - 1998
Externally publishedYes


ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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