Abstract
An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90% identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 345-353 |
| Number of pages | 9 |
| Journal | Acta Microbiologica Polonica |
| Volume | 47 |
| Issue number | 4 |
| Publication status | Published - 1998 Dec 1 |
| Externally published | Yes |
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology