Cloning, Sequencing and Characterisation of a Downstream Region of ksdDI Operon of Arthrobacter simplex

Jarosław Dziadek, Mitsuo Yamashita, Yoshikatsu Murooka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90% identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalActa Microbiologica Polonica
Volume47
Issue number4
Publication statusPublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Cloning, Sequencing and Characterisation of a Downstream Region of ksdDI Operon of Arthrobacter simplex. / Dziadek, Jarosław; Yamashita, Mitsuo; Murooka, Yoshikatsu.

In: Acta Microbiologica Polonica, Vol. 47, No. 4, 1998, p. 345-353.

Research output: Contribution to journalArticle

@article{cac7e2cf353448d2a28bcd0e515e2c23,
title = "Cloning, Sequencing and Characterisation of a Downstream Region of ksdDI Operon of Arthrobacter simplex",
abstract = "An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90{\%} identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed.",
author = "Jarosław Dziadek and Mitsuo Yamashita and Yoshikatsu Murooka",
year = "1998",
language = "English",
volume = "47",
pages = "345--353",
journal = "Polish Journal of Microbiology",
issn = "1733-1331",
publisher = "Polish Society of Microbiologists",
number = "4",

}

TY - JOUR

T1 - Cloning, Sequencing and Characterisation of a Downstream Region of ksdDI Operon of Arthrobacter simplex

AU - Dziadek, Jarosław

AU - Yamashita, Mitsuo

AU - Murooka, Yoshikatsu

PY - 1998

Y1 - 1998

N2 - An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90% identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed.

AB - An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90% identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0032248628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032248628&partnerID=8YFLogxK

M3 - Article

VL - 47

SP - 345

EP - 353

JO - Polish Journal of Microbiology

JF - Polish Journal of Microbiology

SN - 1733-1331

IS - 4

ER -