Cloning, Sequencing and Characterisation of a Downstream Region of ksdDI Operon of Arthrobacter simplex

Jarosław Dziadek, Mitsuo Yamashita, Yoshikatsu Murooka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90% identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalActa Microbiologica Polonica
Volume47
Issue number4
Publication statusPublished - 1998 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Cloning, Sequencing and Characterisation of a Downstream Region of ksdDI Operon of Arthrobacter simplex'. Together they form a unique fingerprint.

  • Cite this