Comparison of direct and mediated electron transfer in electrodes with novel fungal flavin adenine dinucleotide glucose dehydrogenase

Kazuya Ishida, Kouhei Orihara, Hitoshi Muguruma, Hisanori Iwasa, Atsunori Hiratsuka, Katsumi Tsuji, Takahide Kishimoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Direct and mediated electron transfer (DET and MET) in enzyme electrodes with a novel flavin adenine dinucleotidedependent glucose dehydrogenase (FAD-GDH) from fungi are compared for the first time. DET is achieved by placing a single-walled carbon nanotube (CNT) between GDH and a flat gold electrode where the CNT is close to FAD within the distance for DET. MET is induced by using a free electron transfer mediator, potassium hexacyanoferrate, and shuttles electrons from FAD to the gold electrode. Cyclic voltammetry shows that the onset potential for glucose response current in DET is smaller than in MET, and that the distinct redox current peak pairs in MET are observed whereas no peaks are found in DET. The chronoamperometry with respect to a glucose biosensor shows that (i) the response in DET is more rapid than in MET; (ii) the current at more than +0.45V in DET is larger than the current at the current-peak potential in MET; (iii) a DET electrode covers the glucose concentration range for clinical requirements and is not susceptible to interfering agents at +0.45 V; and (iv) a DET electrode with the novel fungal FAD-GDH does not affect sensing accuracy in the presence of up to 5 mM xylose, while it often shows a similar response level to glucose with other conventionally used fungus-derived FAD-GDHs. It is concluded that our DET system overcomes the disadvantage of MET.

Original languageEnglish
Pages (from-to)783-787
Number of pages5
JournalAnalytical Sciences
Volume34
Issue number7
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

DEET
Glucose 1-Dehydrogenase
Flavin-Adenine Dinucleotide
Electrodes
Electrons
Glucose
Adenine
Fungi
Gold
Enzyme electrodes
Chronoamperometry
Carbon Nanotubes
Xylose
Single-walled carbon nanotubes (SWCN)
Biosensors
Cyclic voltammetry
Potassium

Keywords

  • Biosensor
  • Direct electron transfer
  • Flavin adenine dinucleotide-dependent glucose dehydrogenase
  • Mediated electron transfer
  • Single-walled carbon nanotube

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Comparison of direct and mediated electron transfer in electrodes with novel fungal flavin adenine dinucleotide glucose dehydrogenase. / Ishida, Kazuya; Orihara, Kouhei; Muguruma, Hitoshi; Iwasa, Hisanori; Hiratsuka, Atsunori; Tsuji, Katsumi; Kishimoto, Takahide.

In: Analytical Sciences, Vol. 34, No. 7, 01.01.2018, p. 783-787.

Research output: Contribution to journalArticle

Ishida, Kazuya ; Orihara, Kouhei ; Muguruma, Hitoshi ; Iwasa, Hisanori ; Hiratsuka, Atsunori ; Tsuji, Katsumi ; Kishimoto, Takahide. / Comparison of direct and mediated electron transfer in electrodes with novel fungal flavin adenine dinucleotide glucose dehydrogenase. In: Analytical Sciences. 2018 ; Vol. 34, No. 7. pp. 783-787.
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