Comparison of extraction of valuable compounds from microalgae by atmospheric pressure plasmas and pulsed electric fields

Katja Zocher, Robert Banaschik, Christian Schulze, Tilo Schulz, Jana Kredl, Camelia Miron, Michael Schmidt, Sabine Mundt, Wolfgang Frey, Juergen F. Kolb

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Microalgae have recently gained interest, not only as source for biofuel, but also as a resource for pharmaceutical and nutritional substances. The bottleneck for extracting these valuable compounds from microalgae is a thick cell wall of high physical and chemical strength. Several extraction techniques are available, but suffer from different disadvantages. Therefore, new technologies are needed, especially those based on processes that will not affect the chemical composition of ingredients. Among these, physical plasma and pulsed electric field (PEF) might be promising. Three different standard methods, microwave, ultrasound, and homogenization, were compared with plasma treatment and PEF. The plasma sources investigated were corona discharges, a plasma jet, a dielectric barrier discharge, spark discharges, and pin-to-liquid discharges. Chlorella vulgaris was chosen as a model organism. To detect successful cell wall rupture, the protein content of the supernatant and pigment concentration after treatment were determined. Scanning electron microscope images were taken to visualize cell wall damage. Microwave and spark discharge treatment were the most successful methods with comparable extracted total protein content in the supernatant. However, spark discharges achieved higher pigment yield than microwave extraction without the thermal degradation of the pigments observed for microwave extraction.

Original languageEnglish
Pages (from-to)273-302
Number of pages30
JournalPlasma Medicine
Volume6
Issue number3-4
DOIs
Publication statusPublished - 2016 Jan 1
Externally publishedYes

Fingerprint

electric sparks
Atmospheric pressure
atmospheric pressure
pigments
Electric sparks
Microwaves
Electric fields
Pigments
Discharge (fluid mechanics)
Plasmas
microwaves
electric fields
Cells
Chlorella
proteins
Proteins
Plasma jets
Plasma sources
electric corona
thermal degradation

Keywords

  • Extraction
  • Microalgae
  • Microwave
  • Plasma
  • Pulsed electric field
  • Spark discharge

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physics and Astronomy(all)

Cite this

Comparison of extraction of valuable compounds from microalgae by atmospheric pressure plasmas and pulsed electric fields. / Zocher, Katja; Banaschik, Robert; Schulze, Christian; Schulz, Tilo; Kredl, Jana; Miron, Camelia; Schmidt, Michael; Mundt, Sabine; Frey, Wolfgang; Kolb, Juergen F.

In: Plasma Medicine, Vol. 6, No. 3-4, 01.01.2016, p. 273-302.

Research output: Contribution to journalArticle

Zocher, K, Banaschik, R, Schulze, C, Schulz, T, Kredl, J, Miron, C, Schmidt, M, Mundt, S, Frey, W & Kolb, JF 2016, 'Comparison of extraction of valuable compounds from microalgae by atmospheric pressure plasmas and pulsed electric fields', Plasma Medicine, vol. 6, no. 3-4, pp. 273-302. https://doi.org/10.1615/PlasmaMed.2017019104
Zocher, Katja ; Banaschik, Robert ; Schulze, Christian ; Schulz, Tilo ; Kredl, Jana ; Miron, Camelia ; Schmidt, Michael ; Mundt, Sabine ; Frey, Wolfgang ; Kolb, Juergen F. / Comparison of extraction of valuable compounds from microalgae by atmospheric pressure plasmas and pulsed electric fields. In: Plasma Medicine. 2016 ; Vol. 6, No. 3-4. pp. 273-302.
@article{7a263d67be41434b99dbbe4baebbd51b,
title = "Comparison of extraction of valuable compounds from microalgae by atmospheric pressure plasmas and pulsed electric fields",
abstract = "Microalgae have recently gained interest, not only as source for biofuel, but also as a resource for pharmaceutical and nutritional substances. The bottleneck for extracting these valuable compounds from microalgae is a thick cell wall of high physical and chemical strength. Several extraction techniques are available, but suffer from different disadvantages. Therefore, new technologies are needed, especially those based on processes that will not affect the chemical composition of ingredients. Among these, physical plasma and pulsed electric field (PEF) might be promising. Three different standard methods, microwave, ultrasound, and homogenization, were compared with plasma treatment and PEF. The plasma sources investigated were corona discharges, a plasma jet, a dielectric barrier discharge, spark discharges, and pin-to-liquid discharges. Chlorella vulgaris was chosen as a model organism. To detect successful cell wall rupture, the protein content of the supernatant and pigment concentration after treatment were determined. Scanning electron microscope images were taken to visualize cell wall damage. Microwave and spark discharge treatment were the most successful methods with comparable extracted total protein content in the supernatant. However, spark discharges achieved higher pigment yield than microwave extraction without the thermal degradation of the pigments observed for microwave extraction.",
keywords = "Extraction, Microalgae, Microwave, Plasma, Pulsed electric field, Spark discharge",
author = "Katja Zocher and Robert Banaschik and Christian Schulze and Tilo Schulz and Jana Kredl and Camelia Miron and Michael Schmidt and Sabine Mundt and Wolfgang Frey and Kolb, {Juergen F.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1615/PlasmaMed.2017019104",
language = "English",
volume = "6",
pages = "273--302",
journal = "Plasma Medicine",
issn = "1947-5764",
publisher = "Begell House Inc.",
number = "3-4",

}

TY - JOUR

T1 - Comparison of extraction of valuable compounds from microalgae by atmospheric pressure plasmas and pulsed electric fields

AU - Zocher, Katja

AU - Banaschik, Robert

AU - Schulze, Christian

AU - Schulz, Tilo

AU - Kredl, Jana

AU - Miron, Camelia

AU - Schmidt, Michael

AU - Mundt, Sabine

AU - Frey, Wolfgang

AU - Kolb, Juergen F.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Microalgae have recently gained interest, not only as source for biofuel, but also as a resource for pharmaceutical and nutritional substances. The bottleneck for extracting these valuable compounds from microalgae is a thick cell wall of high physical and chemical strength. Several extraction techniques are available, but suffer from different disadvantages. Therefore, new technologies are needed, especially those based on processes that will not affect the chemical composition of ingredients. Among these, physical plasma and pulsed electric field (PEF) might be promising. Three different standard methods, microwave, ultrasound, and homogenization, were compared with plasma treatment and PEF. The plasma sources investigated were corona discharges, a plasma jet, a dielectric barrier discharge, spark discharges, and pin-to-liquid discharges. Chlorella vulgaris was chosen as a model organism. To detect successful cell wall rupture, the protein content of the supernatant and pigment concentration after treatment were determined. Scanning electron microscope images were taken to visualize cell wall damage. Microwave and spark discharge treatment were the most successful methods with comparable extracted total protein content in the supernatant. However, spark discharges achieved higher pigment yield than microwave extraction without the thermal degradation of the pigments observed for microwave extraction.

AB - Microalgae have recently gained interest, not only as source for biofuel, but also as a resource for pharmaceutical and nutritional substances. The bottleneck for extracting these valuable compounds from microalgae is a thick cell wall of high physical and chemical strength. Several extraction techniques are available, but suffer from different disadvantages. Therefore, new technologies are needed, especially those based on processes that will not affect the chemical composition of ingredients. Among these, physical plasma and pulsed electric field (PEF) might be promising. Three different standard methods, microwave, ultrasound, and homogenization, were compared with plasma treatment and PEF. The plasma sources investigated were corona discharges, a plasma jet, a dielectric barrier discharge, spark discharges, and pin-to-liquid discharges. Chlorella vulgaris was chosen as a model organism. To detect successful cell wall rupture, the protein content of the supernatant and pigment concentration after treatment were determined. Scanning electron microscope images were taken to visualize cell wall damage. Microwave and spark discharge treatment were the most successful methods with comparable extracted total protein content in the supernatant. However, spark discharges achieved higher pigment yield than microwave extraction without the thermal degradation of the pigments observed for microwave extraction.

KW - Extraction

KW - Microalgae

KW - Microwave

KW - Plasma

KW - Pulsed electric field

KW - Spark discharge

UR - http://www.scopus.com/inward/record.url?scp=85020458322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020458322&partnerID=8YFLogxK

U2 - 10.1615/PlasmaMed.2017019104

DO - 10.1615/PlasmaMed.2017019104

M3 - Article

AN - SCOPUS:85020458322

VL - 6

SP - 273

EP - 302

JO - Plasma Medicine

JF - Plasma Medicine

SN - 1947-5764

IS - 3-4

ER -