Composite imaging method for histological image analysis

Mizuho Imai, Akane Takei, Keita Miyamoto, Masanobu Takahashi, Masayuki Nakano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A composite imaging method has been developed that enables the user to directly capture a composite image by one-image capturing. It was experimentally verified that the composite images of bright-field, dark-field, and phase-contrast images can be captured with an arbitrary composition ratio. The difference in pixel values between the captured composite image and the computer composite image was small. This imaging method is realized only by placing below the condenser a masking plate, which can easily be made using Neutral Density filters. Therefore, little additional time and cost are needed. The composite imaging method was applied for extracting Helicobacter pylori in microscopic images of HE-stained gastric histological sections. H pylori is difficult to extract because the colors in H pylori are similar to those in other areas. It is experimentally shown that a composite image of phase-contrast and dark-field images captured using the proposed method improves the accuracy for extracting H pylori.

Original languageEnglish
Title of host publicationProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages3387-3390
Number of pages4
DOIs
Publication statusPublished - 2013
Event2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka
Duration: 2013 Jul 32013 Jul 7

Other

Other2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
CityOsaka
Period13/7/313/7/7

Fingerprint

Image analysis
Imaging techniques
Composite materials
Pixels
Color
Chemical analysis
Costs

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

Imai, M., Takei, A., Miyamoto, K., Takahashi, M., & Nakano, M. (2013). Composite imaging method for histological image analysis. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (pp. 3387-3390). [6610268] https://doi.org/10.1109/EMBC.2013.6610268

Composite imaging method for histological image analysis. / Imai, Mizuho; Takei, Akane; Miyamoto, Keita; Takahashi, Masanobu; Nakano, Masayuki.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 3387-3390 6610268.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Imai, M, Takei, A, Miyamoto, K, Takahashi, M & Nakano, M 2013, Composite imaging method for histological image analysis. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS., 6610268, pp. 3387-3390, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, 13/7/3. https://doi.org/10.1109/EMBC.2013.6610268
Imai M, Takei A, Miyamoto K, Takahashi M, Nakano M. Composite imaging method for histological image analysis. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 3387-3390. 6610268 https://doi.org/10.1109/EMBC.2013.6610268
Imai, Mizuho ; Takei, Akane ; Miyamoto, Keita ; Takahashi, Masanobu ; Nakano, Masayuki. / Composite imaging method for histological image analysis. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. pp. 3387-3390
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