Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization

Hongchun Liu; Akira Yamaguchi; Masayuki Hayashida; Shigeki Matsuo; Hiroaki Misawa

doi:10.1143/JJAP.43.2767

Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization

Hongchun Liu, Akira Yamaguchi, Masayuki Hayashida, Shigeki Matsuo, Hiroaki Misawa

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.

Original language	English
Pages (from-to)	2767-2770
Number of pages	4
Journal	Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume	43
Issue number	5 A
DOIs	https://doi.org/10.1143/JJAP.43.2767
Publication status	Published - 2004 May
Externally published	Yes

Keywords

Au nanoparticles
DNA
Hybridization
Multilayer
Surface plasmon resonance (SPR)

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

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10.1143/JJAP.43.2767

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Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization. / Liu, Hongchun; Yamaguchi, Akira; Hayashida, Masayuki et al.

In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 43, No. 5 A, 05.2004, p. 2767-2770.

Research output: Contribution to journal › Article › peer-review

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AU - Misawa, Hiroaki

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N2 - We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.

AB - We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.

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Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization

Abstract

Keywords

ASJC Scopus subject areas

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