Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization

Hongchun Liu; Akira Yamaguchi; Masayuki Hayashida; Shigeki Matsuo; Hiroaki Misawa

doi:10.1143/JJAP.43.2767

Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization

Hongchun Liu, Akira Yamaguchi, Masayuki Hayashida, Shigeki Matsuo, Hiroaki Misawa

Department of Mechanical Engineering

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.

Original language	English
Pages (from-to)	2767-2770
Number of pages	4
Journal	Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume	43
Issue number	5 A
DOIs	https://doi.org/10.1143/JJAP.43.2767
Publication status	Published - 2004 May

Fingerprint

Keywords

Au nanoparticles
DNA
Hybridization
Multilayer
Surface plasmon resonance (SPR)

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

Cite this

Liu, H., Yamaguchi, A., Hayashida, M., Matsuo, S., & Misawa, H. (2004). Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization. Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, 43(5 A), 2767-2770. https://doi.org/10.1143/JJAP.43.2767

Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization. / Liu, Hongchun; Yamaguchi, Akira; Hayashida, Masayuki; Matsuo, Shigeki; Misawa, Hiroaki.

In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 43, No. 5 A, 05.2004, p. 2767-2770.

Research output: Contribution to journal › Article

@article{c1a14fbd92924e6aac7c5649951d8f2b,

title = "Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization",

abstract = "We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.",

keywords = "Au nanoparticles, DNA, Hybridization, Multilayer, Surface plasmon resonance (SPR)",

author = "Hongchun Liu and Akira Yamaguchi and Masayuki Hayashida and Shigeki Matsuo and Hiroaki Misawa",

year = "2004",

month = may

doi = "10.1143/JJAP.43.2767",

language = "English",

volume = "43",

pages = "2767--2770",

journal = "Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes",

issn = "0021-4922",

publisher = "Japan Society of Applied Physics",

number = "5 A",

}

TY - JOUR

T1 - Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization

AU - Liu, Hongchun

AU - Yamaguchi, Akira

AU - Hayashida, Masayuki

AU - Matsuo, Shigeki

AU - Misawa, Hiroaki

PY - 2004/5

Y1 - 2004/5

N2 - We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.

AB - We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.

KW - Au nanoparticles

KW - DNA

KW - Hybridization

KW - Multilayer

KW - Surface plasmon resonance (SPR)

UR - http://www.scopus.com/inward/record.url?scp=3142741723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142741723&partnerID=8YFLogxK

U2 - 10.1143/JJAP.43.2767

DO - 10.1143/JJAP.43.2767

M3 - Article

AN - SCOPUS:3142741723

VL - 43

SP - 2767

EP - 2770

JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

SN - 0021-4922

IS - 5 A

ER -

Access to Document

10.1143/JJAP.43.2767