Abstract
We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.
| Original language | English |
|---|---|
| Pages (from-to) | 2767-2770 |
| Number of pages | 4 |
| Journal | Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers |
| Volume | 43 |
| Issue number | 5 A |
| Publication status | Published - 2004 May |
| Externally published | Yes |
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Keywords
- Au nanoparticles
- DNA
- Hybridization
- Multilayer
- Surface plasmon resonance (SPR)
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
Cite this
Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization. / Liu, Hongchun; Yamaguchi, Akira; Hayashida, Masayuki; Matsuo, Shigeki; Misawa, Hiroaki.
In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 43, No. 5 A, 05.2004, p. 2767-2770.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization
AU - Liu, Hongchun
AU - Yamaguchi, Akira
AU - Hayashida, Masayuki
AU - Matsuo, Shigeki
AU - Misawa, Hiroaki
PY - 2004/5
Y1 - 2004/5
N2 - We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.
AB - We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.
KW - Au nanoparticles
KW - DNA
KW - Hybridization
KW - Multilayer
KW - Surface plasmon resonance (SPR)
UR - http://www.scopus.com/inward/record.url?scp=3142741723&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3142741723&partnerID=8YFLogxK
M3 - Article
VL - 43
SP - 2767
EP - 2770
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
SN - 0021-4922
IS - 5 A
ER -