Construction of peptides with nucleobase amino acids

Design and synthesis of the nucleobase-conjugated peptides derived from HIV-1 rev and their binding properties to HIV-1 RRE RNA

Tsuyoshi Takahashi, Keita Hamasaki, Akihiko Ueno, Hisakazu Mihara

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In order to develop a novel molecule that recognizes a specific structure of RNA, we have attempted to design peptides having L-α-amino acids with a nucleobase at the side chain nucleobase amino acid (NBA), expecting that the function of a nucleobase which can specifically recognize a base in RNA is regulated in a peptide conformation. In this study, to demonstrate the applicability of NBA units in the peptide to RNA recognition, we designed and synthesized a variety of NBA-conjugated peptides, derived from HIV-1 Rev. Circular dichroism study revealed that the conjunction of the Rev peptide with an NBA unit did not disturb the peptide conformation. RNA-binidng affinities of the designed peptides with RRE IIB RNA were dependent on the structure of the nucleobase moieties in the peptides. The peptides having the cytosine NBA at the position of the Asn40 site in the Rev showed a higher binding ability for RRE IIB RNA, despite diminishing the Asn40 function. Furthermore, the peptide having the guanine NBA at the position of the Arg44 site, which is the most important residue for the RNA binding in the Rev, bound to RRE IIB RNA in an ability similar to Rev34-50 with the native sequence. These results demonstrate that an appropriate NBA unit in the peptide plays an important role in the RNA binding with a specific contact such as hydrogen bonding, and the interaction between the nucleobase in the peptide and the base in the RNA can enhance the RNA-binding affinity and specificity.

Original languageEnglish
Pages (from-to)991-1000
Number of pages10
JournalBioorganic and Medicinal Chemistry
Volume9
Issue number4
DOIs
Publication statusPublished - 2001 Apr
Externally publishedYes

Fingerprint

RNA
Amino Acids
Peptides
Conformations
Cytosine
Guanine
Hydrogen bonds
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

@article{b03ed6acea564605b4a74909e70530a8,
title = "Construction of peptides with nucleobase amino acids: Design and synthesis of the nucleobase-conjugated peptides derived from HIV-1 rev and their binding properties to HIV-1 RRE RNA",
abstract = "In order to develop a novel molecule that recognizes a specific structure of RNA, we have attempted to design peptides having L-α-amino acids with a nucleobase at the side chain nucleobase amino acid (NBA), expecting that the function of a nucleobase which can specifically recognize a base in RNA is regulated in a peptide conformation. In this study, to demonstrate the applicability of NBA units in the peptide to RNA recognition, we designed and synthesized a variety of NBA-conjugated peptides, derived from HIV-1 Rev. Circular dichroism study revealed that the conjunction of the Rev peptide with an NBA unit did not disturb the peptide conformation. RNA-binidng affinities of the designed peptides with RRE IIB RNA were dependent on the structure of the nucleobase moieties in the peptides. The peptides having the cytosine NBA at the position of the Asn40 site in the Rev showed a higher binding ability for RRE IIB RNA, despite diminishing the Asn40 function. Furthermore, the peptide having the guanine NBA at the position of the Arg44 site, which is the most important residue for the RNA binding in the Rev, bound to RRE IIB RNA in an ability similar to Rev34-50 with the native sequence. These results demonstrate that an appropriate NBA unit in the peptide plays an important role in the RNA binding with a specific contact such as hydrogen bonding, and the interaction between the nucleobase in the peptide and the base in the RNA can enhance the RNA-binding affinity and specificity.",
author = "Tsuyoshi Takahashi and Keita Hamasaki and Akihiko Ueno and Hisakazu Mihara",
year = "2001",
month = "4",
doi = "10.1016/S0968-0896(00)00324-2",
language = "English",
volume = "9",
pages = "991--1000",
journal = "Bioorganic and Medicinal Chemistry",
issn = "0968-0896",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Construction of peptides with nucleobase amino acids

T2 - Design and synthesis of the nucleobase-conjugated peptides derived from HIV-1 rev and their binding properties to HIV-1 RRE RNA

AU - Takahashi, Tsuyoshi

AU - Hamasaki, Keita

AU - Ueno, Akihiko

AU - Mihara, Hisakazu

PY - 2001/4

Y1 - 2001/4

N2 - In order to develop a novel molecule that recognizes a specific structure of RNA, we have attempted to design peptides having L-α-amino acids with a nucleobase at the side chain nucleobase amino acid (NBA), expecting that the function of a nucleobase which can specifically recognize a base in RNA is regulated in a peptide conformation. In this study, to demonstrate the applicability of NBA units in the peptide to RNA recognition, we designed and synthesized a variety of NBA-conjugated peptides, derived from HIV-1 Rev. Circular dichroism study revealed that the conjunction of the Rev peptide with an NBA unit did not disturb the peptide conformation. RNA-binidng affinities of the designed peptides with RRE IIB RNA were dependent on the structure of the nucleobase moieties in the peptides. The peptides having the cytosine NBA at the position of the Asn40 site in the Rev showed a higher binding ability for RRE IIB RNA, despite diminishing the Asn40 function. Furthermore, the peptide having the guanine NBA at the position of the Arg44 site, which is the most important residue for the RNA binding in the Rev, bound to RRE IIB RNA in an ability similar to Rev34-50 with the native sequence. These results demonstrate that an appropriate NBA unit in the peptide plays an important role in the RNA binding with a specific contact such as hydrogen bonding, and the interaction between the nucleobase in the peptide and the base in the RNA can enhance the RNA-binding affinity and specificity.

AB - In order to develop a novel molecule that recognizes a specific structure of RNA, we have attempted to design peptides having L-α-amino acids with a nucleobase at the side chain nucleobase amino acid (NBA), expecting that the function of a nucleobase which can specifically recognize a base in RNA is regulated in a peptide conformation. In this study, to demonstrate the applicability of NBA units in the peptide to RNA recognition, we designed and synthesized a variety of NBA-conjugated peptides, derived from HIV-1 Rev. Circular dichroism study revealed that the conjunction of the Rev peptide with an NBA unit did not disturb the peptide conformation. RNA-binidng affinities of the designed peptides with RRE IIB RNA were dependent on the structure of the nucleobase moieties in the peptides. The peptides having the cytosine NBA at the position of the Asn40 site in the Rev showed a higher binding ability for RRE IIB RNA, despite diminishing the Asn40 function. Furthermore, the peptide having the guanine NBA at the position of the Arg44 site, which is the most important residue for the RNA binding in the Rev, bound to RRE IIB RNA in an ability similar to Rev34-50 with the native sequence. These results demonstrate that an appropriate NBA unit in the peptide plays an important role in the RNA binding with a specific contact such as hydrogen bonding, and the interaction between the nucleobase in the peptide and the base in the RNA can enhance the RNA-binding affinity and specificity.

UR - http://www.scopus.com/inward/record.url?scp=0035038374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035038374&partnerID=8YFLogxK

U2 - 10.1016/S0968-0896(00)00324-2

DO - 10.1016/S0968-0896(00)00324-2

M3 - Article

VL - 9

SP - 991

EP - 1000

JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

SN - 0968-0896

IS - 4

ER -