Continuous monitoring of development activity changes in cultured cortical networks

Yoshitaka Mukai, Tsuyoshi Shiina, Yasuhiko Jimbo

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


During development, cortical neurons show highly synchronized spontaneous activity. This spontaneous activity probably reflects the mechanisms underlying proper network formation regulated by activity-dependent synaptic modification. To see long-term transitions in this spontaneous activity, we constructed a microelectrode-array (MEA)-based continuous monitoring system. Rat cortical neurons were cultured on MEAs with 64 embedded electrodes and maintained in a conventional CO2 incubator. A perfusion system for medium changes and recording setup for electrical signals were directly connected to the MEA in the incubator. Culture medium was continuously perfused at a very slow rate (0.1 ml/h), which was quite effective at maintaining constant conditions without contamination. Using this system, we succeeded in recording spontaneous activity of cultured cortical networks, almost continuously from 5 days to more than 1 month in vitro. It was revealed that the spontaneous activity patterns showed transition from simple synchronized bursts to a complex mixture of multiple patterns, separated by a brief silent period at approximately 2 weeks in vitro.

Original languageEnglish
Pages (from-to)28-37
Number of pages10
JournalElectrical Engineering in Japan (English translation of Denki Gakkai Ronbunshi)
Issue number4
Publication statusPublished - 2003 Dec
Externally publishedYes


  • Cultured cortical networks
  • Long-term recording
  • MEAs
  • Spike
  • Synchronized burst

ASJC Scopus subject areas

  • Energy Engineering and Power Technology
  • Electrical and Electronic Engineering


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