Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10

Kenichi Iwata, Haruko Noguchi, Yusuke Usami, Jeong Won Nam, Zui Fujimoto, Hiroshi Mizuno, Hiroshi Habe, Hisakazu Yamane, Toshio Omori, Hideaki Nojiri

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P21212, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 Å, α = β = γ = 90°. The crystals diffracted to a maximum resolution of 1.9 Å and gave a data set with an overall R merge of 5.7% and a completeness of 98.6%. The VM value was 2.52 Å3 Da-1, which indicated a solvent content of 51.2%.

Original languageEnglish
Pages (from-to)2340-2342
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume60
Issue number12 II
DOIs
Publication statusPublished - 2004 Dec
Externally publishedYes

Fingerprint

Dioxygenases
pseudomonas
carbazoles
Crystallization
crystallization
Crystals
completeness
Polyethylene glycols
crystals
Vapors
vapors
degradation
Degradation
cells
2-aminodiphenyl
carbazole
extradiol dioxygenase
MME

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10. / Iwata, Kenichi; Noguchi, Haruko; Usami, Yusuke; Nam, Jeong Won; Fujimoto, Zui; Mizuno, Hiroshi; Habe, Hiroshi; Yamane, Hisakazu; Omori, Toshio; Nojiri, Hideaki.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 60, No. 12 II, 12.2004, p. 2340-2342.

Research output: Contribution to journalArticle

Iwata, Kenichi ; Noguchi, Haruko ; Usami, Yusuke ; Nam, Jeong Won ; Fujimoto, Zui ; Mizuno, Hiroshi ; Habe, Hiroshi ; Yamane, Hisakazu ; Omori, Toshio ; Nojiri, Hideaki. / Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10. In: Acta Crystallographica Section D: Biological Crystallography. 2004 ; Vol. 60, No. 12 II. pp. 2340-2342.
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abstract = "CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P21212, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 {\AA}, α = β = γ = 90°. The crystals diffracted to a maximum resolution of 1.9 {\AA} and gave a data set with an overall R merge of 5.7{\%} and a completeness of 98.6{\%}. The VM value was 2.52 {\AA}3 Da-1, which indicated a solvent content of 51.2{\%}.",
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AU - Iwata, Kenichi

AU - Noguchi, Haruko

AU - Usami, Yusuke

AU - Nam, Jeong Won

AU - Fujimoto, Zui

AU - Mizuno, Hiroshi

AU - Habe, Hiroshi

AU - Yamane, Hisakazu

AU - Omori, Toshio

AU - Nojiri, Hideaki

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