Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10

Kenichi Iwata; Haruko Noguchi; Yusuke Usami; Jeong Won Nam; Zui Fujimoto; Hiroshi Mizuno; Hiroshi Habe; Hisakazu Yamane; Toshio Omori; Hideaki Nojiri

doi:10.1107/S0907444904024485

Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10

Kenichi Iwata, Haruko Noguchi, Yusuke Usami, Jeong Won Nam, Zui Fujimoto, Hiroshi Mizuno, Hiroshi Habe, Hisakazu Yamane, Toshio Omori, Hideaki Nojiri

Department of Bioscience and Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P2₁2₁2, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 Å, α = β = γ = 90°. The crystals diffracted to a maximum resolution of 1.9 Å and gave a data set with an overall R _merge of 5.7% and a completeness of 98.6%. The V_M value was 2.52 Å³ Da^-1, which indicated a solvent content of 51.2%.

Original language	English
Pages (from-to)	2340-2342
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	60
Issue number	12 II
DOIs	https://doi.org/10.1107/S0907444904024485
Publication status	Published - 2004 Dec 1

ASJC Scopus subject areas

Structural Biology

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10.1107/S0907444904024485

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Iwata, K., Noguchi, H., Usami, Y., Nam, J. W., Fujimoto, Z., Mizuno, H., Habe, H., Yamane, H., Omori, T., & Nojiri, H. (2004). Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10. Acta Crystallographica Section D: Biological Crystallography, 60(12 II), 2340-2342. https://doi.org/10.1107/S0907444904024485

Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10. / Iwata, Kenichi; Noguchi, Haruko; Usami, Yusuke; Nam, Jeong Won; Fujimoto, Zui; Mizuno, Hiroshi; Habe, Hiroshi; Yamane, Hisakazu; Omori, Toshio; Nojiri, Hideaki.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 60, No. 12 II, 01.12.2004, p. 2340-2342.

Research output: Contribution to journal › Article › peer-review

Iwata, K, Noguchi, H, Usami, Y, Nam, JW, Fujimoto, Z, Mizuno, H, Habe, H, Yamane, H, Omori, T & Nojiri, H 2004, 'Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10', Acta Crystallographica Section D: Biological Crystallography, vol. 60, no. 12 II, pp. 2340-2342. https://doi.org/10.1107/S0907444904024485

Iwata K, Noguchi H, Usami Y, Nam JW, Fujimoto Z, Mizuno H et al. Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10. Acta Crystallographica Section D: Biological Crystallography. 2004 Dec 1;60(12 II):2340-2342. https://doi.org/10.1107/S0907444904024485

Iwata, Kenichi ; Noguchi, Haruko ; Usami, Yusuke ; Nam, Jeong Won ; Fujimoto, Zui ; Mizuno, Hiroshi ; Habe, Hiroshi ; Yamane, Hisakazu ; Omori, Toshio ; Nojiri, Hideaki. / Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10. In: Acta Crystallographica Section D: Biological Crystallography. 2004 ; Vol. 60, No. 12 II. pp. 2340-2342.

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abstract = "CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P21212, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 {\AA}, α = β = γ = 90°. The crystals diffracted to a maximum resolution of 1.9 {\AA} and gave a data set with an overall R merge of 5.7% and a completeness of 98.6%. The VM value was 2.52 {\AA}3 Da-1, which indicated a solvent content of 51.2%.",

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AU - Noguchi, Haruko

AU - Usami, Yusuke

AU - Nam, Jeong Won

AU - Fujimoto, Zui

AU - Mizuno, Hiroshi

AU - Habe, Hiroshi

AU - Yamane, Hisakazu

AU - Omori, Toshio

AU - Nojiri, Hideaki

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