TY - JOUR
T1 - Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10
AU - Iwata, Kenichi
AU - Noguchi, Haruko
AU - Usami, Yusuke
AU - Nam, Jeong Won
AU - Fujimoto, Zui
AU - Mizuno, Hiroshi
AU - Habe, Hiroshi
AU - Yamane, Hisakazu
AU - Omori, Toshio
AU - Nojiri, Hideaki
PY - 2004/12/1
Y1 - 2004/12/1
N2 - CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P21212, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 Å, α = β = γ = 90°. The crystals diffracted to a maximum resolution of 1.9 Å and gave a data set with an overall R merge of 5.7% and a completeness of 98.6%. The VM value was 2.52 Å3 Da-1, which indicated a solvent content of 51.2%.
AB - CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P21212, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 Å, α = β = γ = 90°. The crystals diffracted to a maximum resolution of 1.9 Å and gave a data set with an overall R merge of 5.7% and a completeness of 98.6%. The VM value was 2.52 Å3 Da-1, which indicated a solvent content of 51.2%.
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U2 - 10.1107/S0907444904024485
DO - 10.1107/S0907444904024485
M3 - Article
C2 - 15583384
AN - SCOPUS:21644438267
VL - 60
SP - 2340
EP - 2342
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
SN - 0907-4449
IS - 12 II
ER -