Abstract
CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P21212, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 Å, α = β = γ = 90°. The crystals diffracted to a maximum resolution of 1.9 Å and gave a data set with an overall R merge of 5.7% and a completeness of 98.6%. The VM value was 2.52 Å3 Da-1, which indicated a solvent content of 51.2%.
| Original language | English |
|---|---|
| Pages (from-to) | 2340-2342 |
| Number of pages | 3 |
| Journal | Acta Crystallographica Section D: Biological Crystallography |
| Volume | 60 |
| Issue number | 12 II |
| DOIs | |
| Publication status | Published - 2004 Dec 1 |
ASJC Scopus subject areas
- Structural Biology
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Iwata, K., Noguchi, H., Usami, Y., Nam, J. W., Fujimoto, Z., Mizuno, H., Habe, H., Yamane, H., Omori, T., & Nojiri, H. (2004). Crystallization and preliminary crystallographic analysis of the 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10. Acta Crystallographica Section D: Biological Crystallography, 60(12 II), 2340-2342. https://doi.org/10.1107/S0907444904024485