Crystallization and preliminary X-ray analysis of copper amine oxidase from Escherichia coli K-12

Jung Hyeob Roh, Hideyuki Suzuki, Hidehiko Kumagai, Mitsuo Yamashita, Hiroyuki Azakami, Yoshikatsu Murooka, Bunzo Mikami

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Copper-containing monoamine oxidase (MAO) from Escherichia coli was overproduced in the periplasmic space by expression of the cloned gene. The purified MAO has been crystallized by means of the hanging drop technique using sodium citrate as a precipitant. The crystals belong to the orthorhombic system, space group P212121, with unit cell dimensions of a = 136.1 Å, b = 168.4 Å and c = 81.6 Å. The asymmetric unit contains one molecule of MAO, with a crystal volume per protein mass (V(m)) of 2.88 Å3/Da and a solvent content of 58% by volume. The crystals diffract X-rays to a resolution limit of at least 2.7 Å and are resistant to X-ray radiation damage. They appear to be suitable for X-ray structure analysis.

Original languageEnglish
Pages (from-to)635-637
Number of pages3
JournalJournal of Molecular Biology
Volume238
Issue number4
DOIs
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • Copper-amine oxidase
  • Crystallization
  • Escherichia coli
  • Monoamine oxidase
  • X-ray crystallography

ASJC Scopus subject areas

  • Virology

Cite this

Crystallization and preliminary X-ray analysis of copper amine oxidase from Escherichia coli K-12. / Roh, Jung Hyeob; Suzuki, Hideyuki; Kumagai, Hidehiko; Yamashita, Mitsuo; Azakami, Hiroyuki; Murooka, Yoshikatsu; Mikami, Bunzo.

In: Journal of Molecular Biology, Vol. 238, No. 4, 1994, p. 635-637.

Research output: Contribution to journalArticle

Roh, Jung Hyeob ; Suzuki, Hideyuki ; Kumagai, Hidehiko ; Yamashita, Mitsuo ; Azakami, Hiroyuki ; Murooka, Yoshikatsu ; Mikami, Bunzo. / Crystallization and preliminary X-ray analysis of copper amine oxidase from Escherichia coli K-12. In: Journal of Molecular Biology. 1994 ; Vol. 238, No. 4. pp. 635-637.
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AU - Azakami, Hiroyuki

AU - Murooka, Yoshikatsu

AU - Mikami, Bunzo

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