Cytochrome P450-dependent catabolism of vitamin K: ω-hydroxylation catalyzed by human CYP4F2 and CYP4F11

Katheryne Z. Edson, Bhagwat Prasad, Jashvant D. Unadkat, Yoshitomo Suhara, Toshio Okano, F. Peter Guengerich, Allan E. Rettie

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Vitamin K plays an essential role in many biological processes including blood clotting, maintenance of bone health, and inhibition of arterial calcification. A menaquinone form of vitamin K, MK4, is increasingly recognized for its key roles in mitochondrial electron transport, as a ligand for the nuclear receptor SXR, which controls the expression of genes involved in transport and metabolism of endo-and xenobiotics, and as a pharmacotherapeutic in the treatment of osteoporosis. Although cytochrome P450 (CYP) 4F2 activity is recognized as an important determinant of phylloquinone (K1) metabolism, the enzymes involved in menaquinone catabolism have not been studied previously. CYP4F2 and CYP4F11 were expressed and purified and found to be equally efficient as in vitro catalysts of MK4 ω-hydroxylation. CYP4F2, but not CYP4F11, catalyzed sequential metabolism of MK4 to the ω-acid without apparent release of the intermediate aldehyde. The ω-alcohol could also be metabolized to the acid by microsomal NAD+-dependent alcohol and aldehyde dehydrogenases. LC-MS/MS analysis of trypsinized human liver microsomes (using a surrogate peptide approach) revealed the mean concentrations of CYP4F2 and CYP4F11 to be 14.3 and 8.4 pmol/mg protein, respectively. Microsomal MK4 ω-hydroxylation activities correlated with the CYP4F2 V433M genotype but not the CYP4F11 D446N genotype. Collectively, these data expand the lexicon of vitamin K ω-hydroxylases to include the 'orphan' P450 CYP4F11 and identify a common variant, CYP4F2 (rs2108622), as a major pharmacogenetic variable influencing MK4 catabolism.

Original languageEnglish
Pages (from-to)8276-8285
Number of pages10
JournalBiochemistry
Volume52
Issue number46
DOIs
Publication statusPublished - 2013 Nov 19
Externally publishedYes

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Hydroxylation
Vitamin K
Metabolism
Cytochrome P-450 Enzyme System
Vitamin K 2
Vitamin K 1
Aldehyde Dehydrogenase
Acids
Alcohol Dehydrogenase
Xenobiotics
Cytoplasmic and Nuclear Receptors
Mixed Function Oxygenases
Aldehydes
Liver
NAD
Bone
Blood
Genes
Alcohols
Health

ASJC Scopus subject areas

  • Biochemistry

Cite this

Edson, K. Z., Prasad, B., Unadkat, J. D., Suhara, Y., Okano, T., Peter Guengerich, F., & Rettie, A. E. (2013). Cytochrome P450-dependent catabolism of vitamin K: ω-hydroxylation catalyzed by human CYP4F2 and CYP4F11. Biochemistry, 52(46), 8276-8285. https://doi.org/10.1021/bi401208m

Cytochrome P450-dependent catabolism of vitamin K : ω-hydroxylation catalyzed by human CYP4F2 and CYP4F11. / Edson, Katheryne Z.; Prasad, Bhagwat; Unadkat, Jashvant D.; Suhara, Yoshitomo; Okano, Toshio; Peter Guengerich, F.; Rettie, Allan E.

In: Biochemistry, Vol. 52, No. 46, 19.11.2013, p. 8276-8285.

Research output: Contribution to journalArticle

Edson, KZ, Prasad, B, Unadkat, JD, Suhara, Y, Okano, T, Peter Guengerich, F & Rettie, AE 2013, 'Cytochrome P450-dependent catabolism of vitamin K: ω-hydroxylation catalyzed by human CYP4F2 and CYP4F11', Biochemistry, vol. 52, no. 46, pp. 8276-8285. https://doi.org/10.1021/bi401208m
Edson, Katheryne Z. ; Prasad, Bhagwat ; Unadkat, Jashvant D. ; Suhara, Yoshitomo ; Okano, Toshio ; Peter Guengerich, F. ; Rettie, Allan E. / Cytochrome P450-dependent catabolism of vitamin K : ω-hydroxylation catalyzed by human CYP4F2 and CYP4F11. In: Biochemistry. 2013 ; Vol. 52, No. 46. pp. 8276-8285.
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