Decreased expression levels of cell cycle regulators and matrix metalloproteinases in melanoma from RET-transgenic mice by single irradiation of non-equilibrium atmospheric pressure plasmas

Machiko Iida, Yasuhiro Omata, Chihiro Nakano, Ichiro Yajima, Toyonori Tsuzuki, Kenji Ishikawa, Masaru Hori, Masashi Kato

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8 Citations (Scopus)


Since effective therapies for melanoma with BRAFV600E mutation are being developed, interest has been shown in the development of therapies for melanoma without BRAFV600E mutation. Recently, interest has also been shown in medical application of non-nequilibrium atmospheric pressure plasmas (NEAPPs). We previously suggested that repeated NEAPP irradiation to spontaneously developed benign melanocytic tumors in RFP-RET-transgenic mice (RET-mice) not only suppresses tumor growth but also prevents malignant transformation. In this study, we first confirmed that transcript expression levels of tumor growth regulators (CyclinD1, D2, E1, E2, G2 and PCNA but not CyclinG1) and tumor invasion regulators [Matrix metalloproteinase (MMP)-2, -9 and -14 and melanoma cell adhesion molecule (MCAM)] in melanomas were significantly higher than those in benign melanocytic tumors in RET-mice. We then showed that transcript expression levels of CyclinE1, G1 and G2 and MMP-2 and -9 in melanomas from RET-mice were significantly decreased by single NEAPP irradiation, whereas transcript expression levels of CyclinD1, D2, E2, PCNA, MCAM and MMP-14 were comparable in untreated and NEAPP-treated melanomas. Since no BrafV600E mutation melanomas have been found in RET-mice, our results suggest that single NEAPP irradiation is a potential therapeutic tool for melanoma without BRAFV600E mutation through modulation of the expression levels of tumor growth and invasion regulators.

Original languageEnglish
Pages (from-to)9326-9331
Number of pages6
JournalInternational Journal of Clinical and Experimental Pathology
Issue number8
Publication statusPublished - 2015 Jan 1
Externally publishedYes



  • Invasion
  • Melanoma
  • Non-equilibrium atmospheric pressure plasmas
  • Tumor growth

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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