Deformability of red blood cells and its relation to blood trauma in rotary blood pumps

Nobuo Watanabe, Daisuke Sakota, Katsuhiro Ohuchi, Setsuo Takatani

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In this study, mechanical trauma to red blood cells was evaluated by conventional hemolysis test and a newly developed cyclically reversing shear flow generator. The fresh porcine blood obtained from a local slaughterhouse was subjected to the conventional hemolysis test using a commercial centrifugal blood pump for the duration of 8 h. The measurements consisted of (i) plasma-free hemoglobin based on the standard optical measurement and (ii) the deformability of red blood cells (RBCs) using a cyclically reversing shear flow generator and microscope image acquisition system. The deformability of RBCs was expressed by the L/W value where L and W were the longer and shorter axes of the elongated RBCs' images. Although the plasma-free hemoglobin level increased with the pumping duration, the L/W remained unchanged for the duration of 8 h of pumping to indicate no alteration in the deformability. It was speculated that (i) although RBCs might have been circulated for so many times through the test pump, after each exposure to mechanical stress, RBCs might have recovered, and net effect due to shear stress-exposure time might have been small; and (ii) RBCs' deformability might be maintained near normal until sudden burst or membrane rupture, or the hemoglobin might have continuously leaked through the pores of the thinned membrane created by the mechanical stress. The deformability testing under a fluctuating shear flow could be a new method to quantify subhemolytic mechanical damage that has been accumulated in the RBCs' membrane and that may not be assessed by the conventional hemolysis test.

Original languageEnglish
Pages (from-to)352-358
Number of pages7
JournalArtificial Organs
Volume31
Issue number5
DOIs
Publication statusPublished - 2007 May
Externally publishedYes

Fingerprint

Formability
Blood
Cells
Pumps
Hemoglobin
Shear flow
Hemoglobins
Membranes
Plasmas
Image acquisition
Cell membranes
Shear stress
Microscopes
Testing

Keywords

  • Deformability
  • Fluctuating shear stress
  • Hemocompatibility evaluation
  • Hemolysis test
  • Oscillatory quasi-Couette flow
  • Subhemolytic trauma

ASJC Scopus subject areas

  • Biophysics

Cite this

Deformability of red blood cells and its relation to blood trauma in rotary blood pumps. / Watanabe, Nobuo; Sakota, Daisuke; Ohuchi, Katsuhiro; Takatani, Setsuo.

In: Artificial Organs, Vol. 31, No. 5, 05.2007, p. 352-358.

Research output: Contribution to journalArticle

Watanabe, Nobuo ; Sakota, Daisuke ; Ohuchi, Katsuhiro ; Takatani, Setsuo. / Deformability of red blood cells and its relation to blood trauma in rotary blood pumps. In: Artificial Organs. 2007 ; Vol. 31, No. 5. pp. 352-358.
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