Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies

Keijiro Nakanishi, Adachi Masao, Yoshihiko Sako, Yuzaburo Ishida, Hitoshi Muguruma, Isao Karube

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In this paper, the determination of Alexandrium affine in sea water was performed using piezoelectric immunosensors. In addition, we report a novel method of immobilizing antibodies on the quartz crystal immunosensors using plasma-polymerized ethylenediamine film. The films formed on the quartz crystals are extremely thin, homogeneous and incorporate amino groups. Sensors produced using this method show higher sensitivity than sensors made using conventional immobilization methods for example polyethylenimine or γ-aminopropyltrimethoxysilane.

Original languageEnglish
Pages (from-to)1247-1258
Number of pages12
JournalAnalytical Letters
Volume29
Issue number8
Publication statusPublished - 1996
Externally publishedYes

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Keywords

  • Alexandrium affine
  • IgM
  • Monoclonal antibody
  • Piezoelectric crystal
  • Plasma-polymerized Films

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Analytical Chemistry

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