Determination of lactate by sequential injection analysis using a fluoride ion-selective electrode detector

Yuichi Takahashi, Takashi Masadome

Research output: Contribution to journalArticlepeer-review

Abstract

A combination of sequential Injection Analysis (SIA) system and the ion-selective electrode (ISE) detector is considered to be one of a very effective analytical method. Reaction of lactate with lactate oxidase produces pyruvic acid and hydrogen peroxide. When the produced hydrogen peroxide is reacted with 4-aminoantipyrine (4-AAP) and 4-fluorophenol (4-FP) in the presence of horseradish peroxidase (HRP), F ion is produced. The concentration of lactate can be indirectly determined by measuring the F ion by F ISE. Based on this principle of detection of lactate, a new measurement method of lactate using an SIA with ISE detector was developed.

Original languageEnglish
Pages (from-to)522-524
Number of pages3
JournalDenki Kagaku
Volume88
Issue number6
DOIs
Publication statusPublished - 2020 Nov 5

Keywords

  • Ion-selective Electrode
  • Lactate
  • Sequential Injection Analysis

ASJC Scopus subject areas

  • Electrochemistry

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