Abstract
This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.
| Language | English |
|---|---|
| Pages | 863-867 |
| Number of pages | 5 |
| Journal | Analytical Sciences |
| Volume | 33 |
| Issue number | 7 |
| DOIs |
|
| State | Published - 2017 |
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Keywords
- LC-MS/MS
- Menadione
- Pseudo MRM
- Vitamin K
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring. / Kamao, Maya; Hirota, Yoshihisa; Suhara, Yoshitomo; Tsugawa, Naoko; Nakagawa, Kimie; Okano, Toshio; Hasegawa, Hiroshi.
In: Analytical Sciences, Vol. 33, No. 7, 2017, p. 863-867.Research output: Contribution to journal › Comment/debate
}
TY - JOUR
T1 - Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring
AU - Kamao,Maya
AU - Hirota,Yoshihisa
AU - Suhara,Yoshitomo
AU - Tsugawa,Naoko
AU - Nakagawa,Kimie
AU - Okano,Toshio
AU - Hasegawa,Hiroshi
PY - 2017
Y1 - 2017
N2 - This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.
AB - This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.
KW - LC-MS/MS
KW - Menadione
KW - Pseudo MRM
KW - Vitamin K
UR - http://www.scopus.com/inward/record.url?scp=85021963828&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85021963828&partnerID=8YFLogxK
U2 - 10.2116/analsci.33.863
DO - 10.2116/analsci.33.863
M3 - Comment/debate
VL - 33
SP - 863
EP - 867
JO - Analytical Sciences
T2 - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 7
ER -