Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring

Maya Kamao, Yoshihisa Hirota, Yoshitomo Suhara, Naoko Tsugawa, Kimie Nakagawa, Toshio Okano, Hiroshi Hasegawa

Research output: Research - peer-reviewComment/debate

Abstract

This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

LanguageEnglish
Pages863-867
Number of pages5
JournalAnalytical Sciences
Volume33
Issue number7
DOIs
StatePublished - 2017

Fingerprint

Vitamin K 3
Liquid chromatography
Mass spectrometry
Monitoring
Assays
Ions
Deuterium
Culture Media
Plasmas
Recovery

Keywords

  • LC-MS/MS
  • Menadione
  • Pseudo MRM
  • Vitamin K

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring. / Kamao, Maya; Hirota, Yoshihisa; Suhara, Yoshitomo; Tsugawa, Naoko; Nakagawa, Kimie; Okano, Toshio; Hasegawa, Hiroshi.

In: Analytical Sciences, Vol. 33, No. 7, 2017, p. 863-867.

Research output: Research - peer-reviewComment/debate

Kamao, Maya ; Hirota, Yoshihisa ; Suhara, Yoshitomo ; Tsugawa, Naoko ; Nakagawa, Kimie ; Okano, Toshio ; Hasegawa, Hiroshi. / Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring. In: Analytical Sciences. 2017 ; Vol. 33, No. 7. pp. 863-867
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AU - Kamao,Maya

AU - Hirota,Yoshihisa

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AU - Tsugawa,Naoko

AU - Nakagawa,Kimie

AU - Okano,Toshio

AU - Hasegawa,Hiroshi

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N2 - This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

AB - This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

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