Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring

Maya Kamao; Yoshihisa Hirota; Yoshitomo Suhara; Naoko Tsugawa; Kimie Nakagawa; Toshio Okano; Hiroshi Hasegawa

doi:10.2116/analsci.33.863

Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring

Maya Kamao, Yoshihisa Hirota, Yoshitomo Suhara, Naoko Tsugawa, Kimie Nakagawa, Toshio Okano, Hiroshi Hasegawa

Research output: Contribution to journal › Comment/debate › peer-review

4 Citations (Scopus)

Abstract

This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

Original language	English
Pages (from-to)	863-867
Number of pages	5
Journal	Analytical Sciences
Volume	33
Issue number	7
DOIs	https://doi.org/10.2116/analsci.33.863
Publication status	Published - 2017

Keywords

LC-MS/MS
Menadione
Pseudo MRM
Vitamin K

ASJC Scopus subject areas

Analytical Chemistry

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Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring. / Kamao, Maya; Hirota, Yoshihisa ; Suhara, Yoshitomo; Tsugawa, Naoko; Nakagawa, Kimie; Okano, Toshio; Hasegawa, Hiroshi.

In: Analytical Sciences, Vol. 33, No. 7, 2017, p. 863-867.

Research output: Contribution to journal › Comment/debate › peer-review

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title = "Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring",

abstract = "This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.",

keywords = "LC-MS/MS, Menadione, Pseudo MRM, Vitamin K",

author = "Maya Kamao and Yoshihisa Hirota and Yoshitomo Suhara and Naoko Tsugawa and Kimie Nakagawa and Toshio Okano and Hiroshi Hasegawa",

note = "Funding Information: We thank Madoka Ogata, Mizusa Inui, Akihiro Hamana, Manaka Hayashi, Yuki Kamiya, Syunsuke Sakata, and Mari Nagao for their excellent technical assistance. This work was partly supported by a Grant-in-aid for Challenging Exploratory Research (Grant No. 26560072) from the Japan Society for the Promotion of Science (JSPS).",

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AU - Kamao, Maya

AU - Hirota, Yoshihisa

AU - Suhara, Yoshitomo

AU - Tsugawa, Naoko

AU - Nakagawa, Kimie

AU - Okano, Toshio

AU - Hasegawa, Hiroshi

N1 - Funding Information: We thank Madoka Ogata, Mizusa Inui, Akihiro Hamana, Manaka Hayashi, Yuki Kamiya, Syunsuke Sakata, and Mari Nagao for their excellent technical assistance. This work was partly supported by a Grant-in-aid for Challenging Exploratory Research (Grant No. 26560072) from the Japan Society for the Promotion of Science (JSPS).

PY - 2017

Y1 - 2017

N2 - This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

AB - This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

KW - LC-MS/MS

KW - Menadione

KW - Pseudo MRM

KW - Vitamin K

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