Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring

Maya Kamao, Yoshihisa Hirota, Yoshitomo Suhara, Naoko Tsugawa, Kimie Nakagawa, Toshio Okano, Hiroshi Hasegawa

Research output: Contribution to journalComment/debate

4 Citations (Scopus)

Abstract

This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

Original languageEnglish
Pages (from-to)863-867
Number of pages5
JournalAnalytical Sciences
Volume33
Issue number7
DOIs
Publication statusPublished - 2017

Keywords

  • LC-MS/MS
  • Menadione
  • Pseudo MRM
  • Vitamin K

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring'. Together they form a unique fingerprint.

  • Cite this