Abstract
An analytical method for measuring rosmarinic acid (RA) and luteolin (Lut), the major polyphenolic substances in Perilla frutescens Britton (Labiatae) was established. High-performance liquid chromatography was used to determine the concentration of RA in perilla leaf solutions prepared by two successive extractions with water-acetone-hydrochloric acid (20:80:1, v/v/v). The concentration of Lut in solutions prepared by two successive extractions with ethanol-hydrochloric acid (100:1, v/v) was determined by liquid chromatography-mass spectrometry. The mean concentrations of RA and Lut in the same samples of perilla leaves were 3.95 ± 0.42 and 0.039 ± 0.004 mg·g-1, respectively, with the concentration of RA being 100 times greater than that of Lut. It was also demonstrated that there were seasonal changes in the RA concentration of perilla leaves collected from the same field and that RA levels varied between different growing areas.
| Original language | English |
|---|---|
| Pages (from-to) | 897-901 |
| Number of pages | 5 |
| Journal | Journal of the Science of Food and Agriculture |
| Volume | 86 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2006 Apr 30 |
| Externally published | Yes |
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Keywords
- High-performance liquid chromatography
- Liquid chromatography-mass spectrometry
- Luteolin
- Perilla frutescens Britton
- Rosmarinic acid
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)
Cite this
Determination of rosmarinic acid and luteolin in Perilla frutescens Britton (Labiatae). / Natsume, Midori; Muto, Yuko; Fukuda, Kumiko; Tokunaga, Takahisa; Osakabe, Naomi.
In: Journal of the Science of Food and Agriculture, Vol. 86, No. 6, 30.04.2006, p. 897-901.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of rosmarinic acid and luteolin in Perilla frutescens Britton (Labiatae)
AU - Natsume, Midori
AU - Muto, Yuko
AU - Fukuda, Kumiko
AU - Tokunaga, Takahisa
AU - Osakabe, Naomi
PY - 2006/4/30
Y1 - 2006/4/30
N2 - An analytical method for measuring rosmarinic acid (RA) and luteolin (Lut), the major polyphenolic substances in Perilla frutescens Britton (Labiatae) was established. High-performance liquid chromatography was used to determine the concentration of RA in perilla leaf solutions prepared by two successive extractions with water-acetone-hydrochloric acid (20:80:1, v/v/v). The concentration of Lut in solutions prepared by two successive extractions with ethanol-hydrochloric acid (100:1, v/v) was determined by liquid chromatography-mass spectrometry. The mean concentrations of RA and Lut in the same samples of perilla leaves were 3.95 ± 0.42 and 0.039 ± 0.004 mg·g-1, respectively, with the concentration of RA being 100 times greater than that of Lut. It was also demonstrated that there were seasonal changes in the RA concentration of perilla leaves collected from the same field and that RA levels varied between different growing areas.
AB - An analytical method for measuring rosmarinic acid (RA) and luteolin (Lut), the major polyphenolic substances in Perilla frutescens Britton (Labiatae) was established. High-performance liquid chromatography was used to determine the concentration of RA in perilla leaf solutions prepared by two successive extractions with water-acetone-hydrochloric acid (20:80:1, v/v/v). The concentration of Lut in solutions prepared by two successive extractions with ethanol-hydrochloric acid (100:1, v/v) was determined by liquid chromatography-mass spectrometry. The mean concentrations of RA and Lut in the same samples of perilla leaves were 3.95 ± 0.42 and 0.039 ± 0.004 mg·g-1, respectively, with the concentration of RA being 100 times greater than that of Lut. It was also demonstrated that there were seasonal changes in the RA concentration of perilla leaves collected from the same field and that RA levels varied between different growing areas.
KW - High-performance liquid chromatography
KW - Liquid chromatography-mass spectrometry
KW - Luteolin
KW - Perilla frutescens Britton
KW - Rosmarinic acid
UR - http://www.scopus.com/inward/record.url?scp=33646253423&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646253423&partnerID=8YFLogxK
U2 - 10.1002/jsfa.2432
DO - 10.1002/jsfa.2432
M3 - Article
VL - 86
SP - 897
EP - 901
JO - Journal of the Science of Food and Agriculture
JF - Journal of the Science of Food and Agriculture
SN - 0022-5142
IS - 6
ER -