Development of a continuous system for monitoring endotoxin concentration in dialysate fluid

K. Iijima, T. Miyasaka, Yasuo Yoshimi, K. Sakai, J. A. Aketagawa, H. Tamura, S. Tanaka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A stopped flow type system consisting of a single tube for monitoring endotoxin by the limulus test was developed. In this system sample solution reacted with limulus reagent without contact with outer atmosphere. The objective of the present study is to obtain a sensitive system for monitoring endotoxin concentration ranging under 100 Eu/l in a short time. Peak area of absorbance observed as a function of reaction time at 405 nm was similar to absorbance observed with batch reaction. Short time monitoring of endotoxin concentration ranging above 40 EU/l at a reaction time of 20 min and 80 EU/l at 10 min was possible.

Original languageEnglish
Pages (from-to)524-527
Number of pages4
JournalJapanese Journal of Artificial Organs
Volume27
Issue number2
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • Dialysate
  • Endotoxin
  • Limulus test
  • Stopped flow

ASJC Scopus subject areas

  • Biophysics

Cite this

Iijima, K., Miyasaka, T., Yoshimi, Y., Sakai, K., Aketagawa, J. A., Tamura, H., & Tanaka, S. (1998). Development of a continuous system for monitoring endotoxin concentration in dialysate fluid. Japanese Journal of Artificial Organs, 27(2), 524-527.

Development of a continuous system for monitoring endotoxin concentration in dialysate fluid. / Iijima, K.; Miyasaka, T.; Yoshimi, Yasuo; Sakai, K.; Aketagawa, J. A.; Tamura, H.; Tanaka, S.

In: Japanese Journal of Artificial Organs, Vol. 27, No. 2, 1998, p. 524-527.

Research output: Contribution to journalArticle

Iijima, K, Miyasaka, T, Yoshimi, Y, Sakai, K, Aketagawa, JA, Tamura, H & Tanaka, S 1998, 'Development of a continuous system for monitoring endotoxin concentration in dialysate fluid', Japanese Journal of Artificial Organs, vol. 27, no. 2, pp. 524-527.
Iijima, K. ; Miyasaka, T. ; Yoshimi, Yasuo ; Sakai, K. ; Aketagawa, J. A. ; Tamura, H. ; Tanaka, S. / Development of a continuous system for monitoring endotoxin concentration in dialysate fluid. In: Japanese Journal of Artificial Organs. 1998 ; Vol. 27, No. 2. pp. 524-527.
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