Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation

Yuichi Tada, Kimio Sumaru, Mitsuyoshi Kameda, Katsuhide Ohi, Toshiyuki Takagi, Toshiyuki Kanamori, Yasuo Yoshimi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350-400 nm, 35 mW/cm2, 5 min) even on the region where living cells exist. Cell adhesion was evaluated by observing BALB/3T3 fibroblasts, which had been seeded on PRCS and then irradiated with UV light regionally, after 12 h incubation and subsequent washing to remove scarcely adhering cells. As a result, it was confirmed that the number of remaining cells in irradiated region was at most 2.5 times greater than that in nonirradiated regions, suggesting the implementation of a novel scheme to manipulate living cells individually by light irradiation in a parallel and simultaneous manner. Influence of the composition of the polymer material consisting the substrate surface was also investigated systematically.

Original languageEnglish
Pages (from-to)495-499
Number of pages5
JournalJournal of Applied Polymer Science
Volume100
Issue number1
DOIs
Publication statusPublished - 2006 Apr 5

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Keywords

  • Cell adhesion
  • Cell culture
  • Light irradiation
  • Nitrospirobenzopyran
  • Photo-response

ASJC Scopus subject areas

  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry

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