Development of an electrochemiluminescence flow cell for continuous measurement of antibody concentration in body fluids.

N. Himi, Yasuo Yoshimi, K. Sakai

Research output: Contribution to journalArticle

Abstract

To monitor the changes in the concentration of antibody produced in body fluids due to infection, we developed a new method for continuous measuring of the antibody concentration. Conventional immunoassay is applicable to heterogeneous reaction between antigen and antibody, and is unavailable for continuous measurement. We used the technique of electrochemiluminescence (ECL) to measure antibody concentration continuously in a homogeneous reaction system. We used human serum albumin (HSA) as antigen and luminol as luminous substance. ECL reaction occurred on electrically conductive glass electrode the electric potential of which was kept constant at -0.70 V vs saturated calomel electrode (SCE). In conclusion, antibody concentration can be measured continuously by the ECL reaction which is a promising technique of measurement of antibody specifically reacting with antigen.

Original languageEnglish
Pages (from-to)523-526
Number of pages4
JournalJapanese Journal of Artificial Organs
Volume23
Issue number2
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • antibody
  • electrochemiluminescence (ECL)
  • homogeneous reaction
  • human serum albumin (HSA)
  • luminol
  • monitoring

ASJC Scopus subject areas

  • Biophysics

Cite this

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abstract = "To monitor the changes in the concentration of antibody produced in body fluids due to infection, we developed a new method for continuous measuring of the antibody concentration. Conventional immunoassay is applicable to heterogeneous reaction between antigen and antibody, and is unavailable for continuous measurement. We used the technique of electrochemiluminescence (ECL) to measure antibody concentration continuously in a homogeneous reaction system. We used human serum albumin (HSA) as antigen and luminol as luminous substance. ECL reaction occurred on electrically conductive glass electrode the electric potential of which was kept constant at -0.70 V vs saturated calomel electrode (SCE). In conclusion, antibody concentration can be measured continuously by the ECL reaction which is a promising technique of measurement of antibody specifically reacting with antigen.",
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T1 - Development of an electrochemiluminescence flow cell for continuous measurement of antibody concentration in body fluids.

AU - Himi, N.

AU - Yoshimi, Yasuo

AU - Sakai, K.

PY - 1994

Y1 - 1994

N2 - To monitor the changes in the concentration of antibody produced in body fluids due to infection, we developed a new method for continuous measuring of the antibody concentration. Conventional immunoassay is applicable to heterogeneous reaction between antigen and antibody, and is unavailable for continuous measurement. We used the technique of electrochemiluminescence (ECL) to measure antibody concentration continuously in a homogeneous reaction system. We used human serum albumin (HSA) as antigen and luminol as luminous substance. ECL reaction occurred on electrically conductive glass electrode the electric potential of which was kept constant at -0.70 V vs saturated calomel electrode (SCE). In conclusion, antibody concentration can be measured continuously by the ECL reaction which is a promising technique of measurement of antibody specifically reacting with antigen.

AB - To monitor the changes in the concentration of antibody produced in body fluids due to infection, we developed a new method for continuous measuring of the antibody concentration. Conventional immunoassay is applicable to heterogeneous reaction between antigen and antibody, and is unavailable for continuous measurement. We used the technique of electrochemiluminescence (ECL) to measure antibody concentration continuously in a homogeneous reaction system. We used human serum albumin (HSA) as antigen and luminol as luminous substance. ECL reaction occurred on electrically conductive glass electrode the electric potential of which was kept constant at -0.70 V vs saturated calomel electrode (SCE). In conclusion, antibody concentration can be measured continuously by the ECL reaction which is a promising technique of measurement of antibody specifically reacting with antigen.

KW - antibody

KW - electrochemiluminescence (ECL)

KW - homogeneous reaction

KW - human serum albumin (HSA)

KW - luminol

KW - monitoring

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