Development of an electrochemiluminescent(ECL) immunosensor; Determination of antibody concentration and electrochemiluminescence mechanism

H. Haramoto, Yasuo Yoshimi, K. Sakai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We designed a technique of continuous and simple immunoassay using an electrochemiluminescence flow cell for monitoring antibody or antigen concentration as an indication of optimal amount of immunosupressors, and attempted to clarify enhanced ECL intensity produced by the binding of antigen and antibody. Luminol-labeled antigens were used to measure the concentration of these antibodies. Luminol-labeled anti-human serum albumin(HSA) antibody was used to measure HSA concentration. Both anti-HSA antibody and anti-immunoglobulin G antibody ranging from 0 to 22 mg/ml, 0 to 200 μg/ml, respectively, increased the ECL intensity of aqueous luminol- labeled antigens HSA ranging from 0 to 38 μM also increased the ECL of luminol-labeled anti-HSA antibody. The ECL intensity of luminol-labeled antigen also increased with antibody concentration in bovine plasma. These results demonstrate that this system is promising for the homogeneous immunoassay of antibodies or antigens and the principal reason of ECL intensity enhancement is an increase in quantum yield of luminol caused by the binding of antigen and antibody.

Original languageEnglish
Pages (from-to)683-687
Number of pages5
JournalJapanese Journal of Artificial Organs
Volume25
Issue number3
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • antibody
  • antigen
  • electrochemiluminescence
  • flow cell
  • luminol

ASJC Scopus subject areas

  • Biophysics

Cite this

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title = "Development of an electrochemiluminescent(ECL) immunosensor; Determination of antibody concentration and electrochemiluminescence mechanism",
abstract = "We designed a technique of continuous and simple immunoassay using an electrochemiluminescence flow cell for monitoring antibody or antigen concentration as an indication of optimal amount of immunosupressors, and attempted to clarify enhanced ECL intensity produced by the binding of antigen and antibody. Luminol-labeled antigens were used to measure the concentration of these antibodies. Luminol-labeled anti-human serum albumin(HSA) antibody was used to measure HSA concentration. Both anti-HSA antibody and anti-immunoglobulin G antibody ranging from 0 to 22 mg/ml, 0 to 200 μg/ml, respectively, increased the ECL intensity of aqueous luminol- labeled antigens HSA ranging from 0 to 38 μM also increased the ECL of luminol-labeled anti-HSA antibody. The ECL intensity of luminol-labeled antigen also increased with antibody concentration in bovine plasma. These results demonstrate that this system is promising for the homogeneous immunoassay of antibodies or antigens and the principal reason of ECL intensity enhancement is an increase in quantum yield of luminol caused by the binding of antigen and antibody.",
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T1 - Development of an electrochemiluminescent(ECL) immunosensor; Determination of antibody concentration and electrochemiluminescence mechanism

AU - Haramoto, H.

AU - Yoshimi, Yasuo

AU - Sakai, K.

PY - 1996

Y1 - 1996

N2 - We designed a technique of continuous and simple immunoassay using an electrochemiluminescence flow cell for monitoring antibody or antigen concentration as an indication of optimal amount of immunosupressors, and attempted to clarify enhanced ECL intensity produced by the binding of antigen and antibody. Luminol-labeled antigens were used to measure the concentration of these antibodies. Luminol-labeled anti-human serum albumin(HSA) antibody was used to measure HSA concentration. Both anti-HSA antibody and anti-immunoglobulin G antibody ranging from 0 to 22 mg/ml, 0 to 200 μg/ml, respectively, increased the ECL intensity of aqueous luminol- labeled antigens HSA ranging from 0 to 38 μM also increased the ECL of luminol-labeled anti-HSA antibody. The ECL intensity of luminol-labeled antigen also increased with antibody concentration in bovine plasma. These results demonstrate that this system is promising for the homogeneous immunoassay of antibodies or antigens and the principal reason of ECL intensity enhancement is an increase in quantum yield of luminol caused by the binding of antigen and antibody.

AB - We designed a technique of continuous and simple immunoassay using an electrochemiluminescence flow cell for monitoring antibody or antigen concentration as an indication of optimal amount of immunosupressors, and attempted to clarify enhanced ECL intensity produced by the binding of antigen and antibody. Luminol-labeled antigens were used to measure the concentration of these antibodies. Luminol-labeled anti-human serum albumin(HSA) antibody was used to measure HSA concentration. Both anti-HSA antibody and anti-immunoglobulin G antibody ranging from 0 to 22 mg/ml, 0 to 200 μg/ml, respectively, increased the ECL intensity of aqueous luminol- labeled antigens HSA ranging from 0 to 38 μM also increased the ECL of luminol-labeled anti-HSA antibody. The ECL intensity of luminol-labeled antigen also increased with antibody concentration in bovine plasma. These results demonstrate that this system is promising for the homogeneous immunoassay of antibodies or antigens and the principal reason of ECL intensity enhancement is an increase in quantum yield of luminol caused by the binding of antigen and antibody.

KW - antibody

KW - antigen

KW - electrochemiluminescence

KW - flow cell

KW - luminol

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VL - 25

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JO - Japanese Journal of Artificial Organs

JF - Japanese Journal of Artificial Organs

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