Abstract
We designed a technique of continuous and simple immunoassay using an electrochemiluminescence flow cell for monitoring antibody or antigen concentration as an indication of optimal amount of immunosupressors, and attempted to clarify enhanced ECL intensity produced by the binding of antigen and antibody. Luminol-labeled antigens were used to measure the concentration of these antibodies. Luminol-labeled anti-human serum albumin(HSA) antibody was used to measure HSA concentration. Both anti-HSA antibody and anti-immunoglobulin G antibody ranging from 0 to 22 mg/ml, 0 to 200 μg/ml, respectively, increased the ECL intensity of aqueous luminol- labeled antigens HSA ranging from 0 to 38 μM also increased the ECL of luminol-labeled anti-HSA antibody. The ECL intensity of luminol-labeled antigen also increased with antibody concentration in bovine plasma. These results demonstrate that this system is promising for the homogeneous immunoassay of antibodies or antigens and the principal reason of ECL intensity enhancement is an increase in quantum yield of luminol caused by the binding of antigen and antibody.
| Original language | English |
|---|---|
| Pages (from-to) | 683-687 |
| Number of pages | 5 |
| Journal | Japanese Journal of Artificial Organs |
| Volume | 25 |
| Issue number | 3 |
| Publication status | Published - 1996 |
| Externally published | Yes |
Keywords
- antibody
- antigen
- electrochemiluminescence
- flow cell
- luminol
ASJC Scopus subject areas
- Biophysics
Cite this
Development of an electrochemiluminescent(ECL) immunosensor; Determination of antibody concentration and electrochemiluminescence mechanism. / Haramoto, H.; Yoshimi, Yasuo; Sakai, K.
In: Japanese Journal of Artificial Organs, Vol. 25, No. 3, 1996, p. 683-687.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of an electrochemiluminescent(ECL) immunosensor; Determination of antibody concentration and electrochemiluminescence mechanism
AU - Haramoto, H.
AU - Yoshimi, Yasuo
AU - Sakai, K.
PY - 1996
Y1 - 1996
N2 - We designed a technique of continuous and simple immunoassay using an electrochemiluminescence flow cell for monitoring antibody or antigen concentration as an indication of optimal amount of immunosupressors, and attempted to clarify enhanced ECL intensity produced by the binding of antigen and antibody. Luminol-labeled antigens were used to measure the concentration of these antibodies. Luminol-labeled anti-human serum albumin(HSA) antibody was used to measure HSA concentration. Both anti-HSA antibody and anti-immunoglobulin G antibody ranging from 0 to 22 mg/ml, 0 to 200 μg/ml, respectively, increased the ECL intensity of aqueous luminol- labeled antigens HSA ranging from 0 to 38 μM also increased the ECL of luminol-labeled anti-HSA antibody. The ECL intensity of luminol-labeled antigen also increased with antibody concentration in bovine plasma. These results demonstrate that this system is promising for the homogeneous immunoassay of antibodies or antigens and the principal reason of ECL intensity enhancement is an increase in quantum yield of luminol caused by the binding of antigen and antibody.
AB - We designed a technique of continuous and simple immunoassay using an electrochemiluminescence flow cell for monitoring antibody or antigen concentration as an indication of optimal amount of immunosupressors, and attempted to clarify enhanced ECL intensity produced by the binding of antigen and antibody. Luminol-labeled antigens were used to measure the concentration of these antibodies. Luminol-labeled anti-human serum albumin(HSA) antibody was used to measure HSA concentration. Both anti-HSA antibody and anti-immunoglobulin G antibody ranging from 0 to 22 mg/ml, 0 to 200 μg/ml, respectively, increased the ECL intensity of aqueous luminol- labeled antigens HSA ranging from 0 to 38 μM also increased the ECL of luminol-labeled anti-HSA antibody. The ECL intensity of luminol-labeled antigen also increased with antibody concentration in bovine plasma. These results demonstrate that this system is promising for the homogeneous immunoassay of antibodies or antigens and the principal reason of ECL intensity enhancement is an increase in quantum yield of luminol caused by the binding of antigen and antibody.
KW - antibody
KW - antigen
KW - electrochemiluminescence
KW - flow cell
KW - luminol
UR - http://www.scopus.com/inward/record.url?scp=0029844480&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029844480&partnerID=8YFLogxK
M3 - Article
VL - 25
SP - 683
EP - 687
JO - Japanese Journal of Artificial Organs
JF - Japanese Journal of Artificial Organs
SN - 0300-0818
IS - 3
ER -