Development of an enzyme activity on-off switchable type glucose sensor using polymeric mediator

A response current may be changed by fouling caused by protein or fibrobrast adsorption when a glucose sensor is implanted, fly making the slope of glucose concentration in an enzyme layer flat, the influence of the fouling layer can be disregarded. In the present studs ferrocene binding with bovine serum albumin was used as a mediator of the glucose sensor. The proportionality between transient current of after activation of glucose oxidase controlled by pulse potential and glucose concentration was examined. As a result reaction right after the activation was not controlled by glucose diffusion, and transient current was dependent on glucose concentration. In conclusion glucose concentration is determinable by measurement of transient current after the activation of glucose oxidase in the enzyme layer, and this method is applicable to long-term monitoring of glucose concentration.