Abstract
We developed a microchip for kinetic analysis of neural response toward chemical stimulation by using rapid electrochemical micropump and realtime voltage imaging. Aplysia neurons stained with a voltage sensitive dye was stimulated by a solution of potassium chloride or acetylcholine administrated by the diaphragm micropump powered by electrolysis. The change in the membrane potential of the neuron was monitored by a fluorescent microscope. The neural response time to acetylcholine was twice as long as that to potassium chloride. The device may be useful for observation of the kinetic effect of therapeutic drugs on neural response to transmitters.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 605-606 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| Publication status | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13 |
Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|---|
| Country | Ireland |
| City | Dublin |
| Period | 16/10/9 → 16/10/13 |
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Keywords
- Brain-on-chip
- Neural signal
- Polydimethylsiloxane
- Voltage sensitive dye imaging
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Development of "brain-on-chip" devise including micropump for rapid administration of transmitters by and voltage imaging system for neurosignal detection. / Harada, K.; Yoshimi, Yasuo.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 605-606.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Development of "brain-on-chip" devise including micropump for rapid administration of transmitters by and voltage imaging system for neurosignal detection
AU - Harada, K.
AU - Yoshimi, Yasuo
PY - 2016
Y1 - 2016
N2 - We developed a microchip for kinetic analysis of neural response toward chemical stimulation by using rapid electrochemical micropump and realtime voltage imaging. Aplysia neurons stained with a voltage sensitive dye was stimulated by a solution of potassium chloride or acetylcholine administrated by the diaphragm micropump powered by electrolysis. The change in the membrane potential of the neuron was monitored by a fluorescent microscope. The neural response time to acetylcholine was twice as long as that to potassium chloride. The device may be useful for observation of the kinetic effect of therapeutic drugs on neural response to transmitters.
AB - We developed a microchip for kinetic analysis of neural response toward chemical stimulation by using rapid electrochemical micropump and realtime voltage imaging. Aplysia neurons stained with a voltage sensitive dye was stimulated by a solution of potassium chloride or acetylcholine administrated by the diaphragm micropump powered by electrolysis. The change in the membrane potential of the neuron was monitored by a fluorescent microscope. The neural response time to acetylcholine was twice as long as that to potassium chloride. The device may be useful for observation of the kinetic effect of therapeutic drugs on neural response to transmitters.
KW - Brain-on-chip
KW - Neural signal
KW - Polydimethylsiloxane
KW - Voltage sensitive dye imaging
UR - http://www.scopus.com/inward/record.url?scp=85014230668&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014230668&partnerID=8YFLogxK
M3 - Conference contribution
SP - 605
EP - 606
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
ER -