Development of xylitol oxidase-based flow injection analysis for monitoring of xylitol concentrations

J. I. Rhee, M. Yamashita, Th Scheper

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A flow injection method for monitoring xylitol was developed using xylitol oxidase (XYO) immobilized on a VA-Epoxy Biosynth E3-support. The immobilized XYO cartridge had a good operational lifetime (up to 24h) and storage stability (up to 1 month). The XYO-FIA system with an oxygen electrode was investigated systematically regarding the factors that can affect enzyme activity, such as pH, reaction temperature, carrier solution and sample matrix. In order to attain high activity of the immobilized XYO, potassium phosphate solution (1M) with 0.5gl-1 Triton X-100 adjusted to pH 8.5 was used as the carrier solution. Sample matrix effects on the immobilized XYO were also investigated. High concentrations of some components (arabinose, 20gl-1; xylose, 30gl-1; NaCl, 30gl-1) in the sample had significant inhibitory effects on the response of the XYO-FIA system. The performance of the XYO-FIA system was tested by using different sample injection volumes (75-250μl) and carrier flow rates (0.7-2.0mlmin-1).

Original languageEnglish
Pages (from-to)293-301
Number of pages9
JournalAnalytica Chimica Acta
Volume456
Issue number2
DOIs
Publication statusPublished - 2002 Apr 8

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Keywords

  • Flow injection analysis
  • Monitoring xylitol
  • Xylitol oxidase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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