Differences in the bone differentiation properties of MC3T3-E1 cells on polished bulk and sputter-deposited titanium specimens

Kei Oya, Yuta Tanaka, Yoshihisa Moriyama, Yuki Yoshioka, Tsuyoshi Kimura, Yusuke Tsutsumi, Hisashi Doi, Naoyuki Nomura, Kazuhiko Noda, Akio Kishida, Takao Hanawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The roughness and cleanness of a titanium surface must be controlled in order to investigate the expression mechanism of hard tissue compatibility on titanium. In this study, osteogenic MC3T3-E1 cells were cultured and differentiation-induced on bulk and sputter-deposited titanium specimens, and the osteogenesis were investigated. For the preparation of bulk specimens, titanium discs were mirrorpolished. On the other hand, titanium was sputter-deposited on smooth and clean cover glasses as sputter-deposited specimens. As a result, no significant difference was observed in the cell morphology and attached number. On the other hand, the time showing maximum activity in the alkaline phosphatase and gene expressions, which are related to bone differentiation on the bulk titanium, were superior to those on the sputter-deposited titanium. From the surface observation of the specimens with a scanning electron microscope and a scanning probe microscope, the surface on the sputter-deposited titanium was more uniform and cleaner than that on the bulk titanium. According to X-ray photoelectron spectroscopy, the thickness of surface oxide film on the sputter-deposited titanium was smaller than that on the bulk titanium. In addition, the proportions of TiO and Ti2O 3 in the surface oxide film on the sputter-deposited titanium were larger than those on the bulk titanium. These differences might influence the differentiation of osteoblastic cells.

Original languageEnglish
Pages (from-to)611-618
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume94
Issue number2
DOIs
Publication statusPublished - 2010 Aug

Fingerprint

Titanium
Bone
Oxide films
Scanning
Phosphatases
Gene expression
Alkaline Phosphatase
Microscopes
Electron microscopes
X ray photoelectron spectroscopy
Surface roughness
Cells
Tissue

Keywords

  • Bone differentiation properties
  • Bulk bodies
  • MC3T3-E1 cells
  • Sputter deposition
  • Titanium

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Differences in the bone differentiation properties of MC3T3-E1 cells on polished bulk and sputter-deposited titanium specimens. / Oya, Kei; Tanaka, Yuta; Moriyama, Yoshihisa; Yoshioka, Yuki; Kimura, Tsuyoshi; Tsutsumi, Yusuke; Doi, Hisashi; Nomura, Naoyuki; Noda, Kazuhiko; Kishida, Akio; Hanawa, Takao.

In: Journal of Biomedical Materials Research - Part A, Vol. 94, No. 2, 08.2010, p. 611-618.

Research output: Contribution to journalArticle

Oya, K, Tanaka, Y, Moriyama, Y, Yoshioka, Y, Kimura, T, Tsutsumi, Y, Doi, H, Nomura, N, Noda, K, Kishida, A & Hanawa, T 2010, 'Differences in the bone differentiation properties of MC3T3-E1 cells on polished bulk and sputter-deposited titanium specimens', Journal of Biomedical Materials Research - Part A, vol. 94, no. 2, pp. 611-618. https://doi.org/10.1002/jbm.a.32751
Oya, Kei ; Tanaka, Yuta ; Moriyama, Yoshihisa ; Yoshioka, Yuki ; Kimura, Tsuyoshi ; Tsutsumi, Yusuke ; Doi, Hisashi ; Nomura, Naoyuki ; Noda, Kazuhiko ; Kishida, Akio ; Hanawa, Takao. / Differences in the bone differentiation properties of MC3T3-E1 cells on polished bulk and sputter-deposited titanium specimens. In: Journal of Biomedical Materials Research - Part A. 2010 ; Vol. 94, No. 2. pp. 611-618.
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