Differential setup of light-addressable potentiometric sensor with an enzyme reactor in a flow channel

Ko Ichiro Miyamoto, Midori Yoshida, Taito Sakai, Atsushi Matsuzaka, Torsten Wagner, Shin'ichiro Kanoh, Tatsuo Yoshinobu, Michael J. Schöning

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Abstract

The light-addressable potentiometric sensor (LAPS) was combined with an enzyme reactor in a fluidic channel. The fluidic channel was mounted on the sensor plate and the enzyme reactor was connected to the fluidic channel. The enzyme reactor was filled with glass beads as enzyme carrier modified by urease which catalyzed production of ammonia depending on the concentration of urea. Double-channel LAPS measurement was performed at the both side of upper and lower stream of the enzyme reactor which realized a differential measurement and eliminated the drift component of the measurement.

Original languageEnglish
Article number04DL08
JournalJapanese Journal of Applied Physics
Volume50
Issue number4 PART 2
DOIs
Publication statusPublished - 2011 Apr 1

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ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Miyamoto, K. I., Yoshida, M., Sakai, T., Matsuzaka, A., Wagner, T., Kanoh, S., Yoshinobu, T., & Schöning, M. J. (2011). Differential setup of light-addressable potentiometric sensor with an enzyme reactor in a flow channel. Japanese Journal of Applied Physics, 50(4 PART 2), [04DL08]. https://doi.org/10.1143/JJAP.50.04DL08