Direct detection of superoxide anion generated in C60-photosensitized oxidation of NADH and an analogue by molecular oxygen

Ikuo Nakanishi, Kei Ohkubo, Shunsuke Fujita, Shunichi Fukuzumi, Toshifumi Konishi, Mamoru Fujitsuka, Osamu Ito, Naoki Miyata

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Abstract

Visible-light irradiation of poly(vinylpyrrolidone) (PVP)-solubilized C60 in water in the presence of NADH (dihydronicotinamide adenine dinucleotide) and molecular oxygen (O2) results in formation of superoxide anion (O2·-). Formation of O2·- having a characteristic g value of 2.18 was evidenced by the direct observation with use of a low-temperature EPR technique at 77 K. Photoinduced O2·- formation was observed for an N-methyl-2-pyrrolidone (NMP) solution of C60 and 1-benzyl-1,4-dihydronicotinamide (BNAH) in the presence of O2, whereas C60 radical anion (C2·-) was formed in the absence of O2 under otherwise the same experimental conditions. These results suggest that C602- formed in the photoinduced electron-transfer reduction of C60 by BNAH acts as an electron donor to O2 to give O2·- in NMP.

Original languageEnglish
Pages (from-to)1829-1833
Number of pages5
JournalJournal of the Chemical Society, Perkin Transactions 2
Issue number11
Publication statusPublished - 2002 Nov 28

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ASJC Scopus subject areas

  • Chemistry(all)

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