Direct determination of p-hydroxymethamphetamine glucuronide in human urine by high-performance liquid chromatography

Rika Nakajima, Muneo Morishita, Sadakazu Aiso, Takehiko Yajima

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High-performance liquid chromatography (HPLC) with UV detection for the simultaneous determination of the free form of p-hydroxymethamphetamine (p-OHMA) and its metabolite, glucuronide (p-OHMAG) was accomplished for the first time. We achieved this by employing 1) an ion pair reagent for retention of sample to a solid-phase extraction (SPE) cartridge, Sep-Pak® Light C18 and 2) a simple two-step stepwise elution technique for subsequent ion pair RP-HPLC. The proposed method was optimized for resolution of p-OHMAG, p-OHMA and MA. The method was successfully applied to urine samples collected from MA abusers.

Original languageEnglish
Pages (from-to)493-495
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Issue number4
Publication statusPublished - 2006 Apr 1



  • HPLC
  • Ion pair reagent
  • Solid-phase extraction
  • Urine sample
  • p-hydroxymethamphetamine glucuronide

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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