Direct observation of immunoreactive sites and antibody molecules by ultrahigh‐resolution scanning electron microscope

Kenjiro Yasuda, Sadakazu Aiso, Takashi Nagatani, Mitsuhiko Yamada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Using an ultrahigh‐resolution scanning electron microscope (SEM), an attempt was made to directly visualize antibody molecules at antigenic sites in rat pancreas or on silicone plates. Although individual antibody molecules could not be discerned, a fluffy meshwork, probably indicating several molecules, was seen. With further improvements in specimen preparation, the high‐resolution SEM promises to be an important tool in examining individual antibody‐antigen sites without the need of an electron‐dense label such as colloidal gold.

Original languageEnglish
Pages (from-to)155-159
Number of pages5
JournalJournal of Electron Microscopy Technique
Volume12
Issue number2
DOIs
Publication statusPublished - 1989 Jan 1
Externally publishedYes

Fingerprint

antibodies
Antibodies
Electron microscopes
electron microscopes
Scanning
Molecules
scanning
Specimen preparation
molecules
pancreas
silicones
Silicones
rats
Rats
Labels
Gold
gold
preparation

Keywords

  • Antibody
  • Colloidal gold
  • Ultrahigh‐resolution SEM

ASJC Scopus subject areas

  • Anatomy

Cite this

Direct observation of immunoreactive sites and antibody molecules by ultrahigh‐resolution scanning electron microscope. / Yasuda, Kenjiro; Aiso, Sadakazu; Nagatani, Takashi; Yamada, Mitsuhiko.

In: Journal of Electron Microscopy Technique, Vol. 12, No. 2, 01.01.1989, p. 155-159.

Research output: Contribution to journalArticle

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