Dose-response study of daily cocoa intake on the oxidative susceptibility of low-density lipoprotein in healthy human volunteers

Naomi Osakabe, Seigo Baba, Akiko Yasuda, Tamami Iwamoto, Masumi Kamiyama, Takahisa Tokunaga, Kazuo Kondo

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17 Citations (Scopus)

Abstract

A four-period crossover study was conducted to evaluate the dose-related response of daily cocoa intake on the oxidative susceptibility of low-density lipoprotein (LDL) in healthy human volunteers. Each supplementation phase consisted of a 14-day feeding period followed by a 28-day washout period. During the period, healthy male volunteers (n = 8) ingested 18, 24 or 36 g of cocoa powder per day (1.3, 1.74 or 2.61 g of polyphenols per day). During the control period, these subjects ingested sugar. LDL oxidative susceptibility was measured as the lag time of conjugated diene formation that started with the addition of a radical initiator, 2,2′-azobis (4-methoxy-2,4-dimethyl-valeronitrile). Samples were analyzed at pre-, one week, and two weeks post-supplementation. In the 24 and 36 g cocoa powder ingestion period, significant lag time prolongation was observed. We conclude that the ingestion of more than 24 g of cocoa powder per day (1.74 g of polyphenols) clearly protects LDL from oxidation.

Original languageEnglish
Pages (from-to)679-684
Number of pages6
JournalJournal of Health Science
Volume50
Issue number6
DOIs
Publication statusPublished - 2004 Dec 1

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Keywords

  • Cocoa
  • Dose-related
  • Low-density lipoprotein
  • Oxidation
  • Polyphenol

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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