Dose-response study of daily cocoa intake on the oxidative susceptibility of low-density lipoprotein in healthy human volunteers

Naomi Osakabe, Seigo Baba, Akiko Yasuda, Tamami Iwamoto, Masumi Kamiyama, Takahisa Tokunaga, Kazuo Kondo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A four-period crossover study was conducted to evaluate the dose-related response of daily cocoa intake on the oxidative susceptibility of low-density lipoprotein (LDL) in healthy human volunteers. Each supplementation phase consisted of a 14-day feeding period followed by a 28-day washout period. During the period, healthy male volunteers (n = 8) ingested 18, 24 or 36 g of cocoa powder per day (1.3, 1.74 or 2.61 g of polyphenols per day). During the control period, these subjects ingested sugar. LDL oxidative susceptibility was measured as the lag time of conjugated diene formation that started with the addition of a radical initiator, 2,2′-azobis (4-methoxy-2,4-dimethyl-valeronitrile). Samples were analyzed at pre-, one week, and two weeks post-supplementation. In the 24 and 36 g cocoa powder ingestion period, significant lag time prolongation was observed. We conclude that the ingestion of more than 24 g of cocoa powder per day (1.74 g of polyphenols) clearly protects LDL from oxidation.

Original languageEnglish
Pages (from-to)679-684
Number of pages6
JournalJournal of Health Science
Volume50
Issue number6
DOIs
Publication statusPublished - 2004 Dec
Externally publishedYes

Fingerprint

Cocoa
LDL Lipoproteins
Powders
Polyphenols
Sugars
Oxidation

Keywords

  • Cocoa
  • Dose-related
  • Low-density lipoprotein
  • Oxidation
  • Polyphenol

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Dose-response study of daily cocoa intake on the oxidative susceptibility of low-density lipoprotein in healthy human volunteers. / Osakabe, Naomi; Baba, Seigo; Yasuda, Akiko; Iwamoto, Tamami; Kamiyama, Masumi; Tokunaga, Takahisa; Kondo, Kazuo.

In: Journal of Health Science, Vol. 50, No. 6, 12.2004, p. 679-684.

Research output: Contribution to journalArticle

Osakabe, Naomi ; Baba, Seigo ; Yasuda, Akiko ; Iwamoto, Tamami ; Kamiyama, Masumi ; Tokunaga, Takahisa ; Kondo, Kazuo. / Dose-response study of daily cocoa intake on the oxidative susceptibility of low-density lipoprotein in healthy human volunteers. In: Journal of Health Science. 2004 ; Vol. 50, No. 6. pp. 679-684.
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