Duramycin-induced destabilization of a phosphatidylethanolamine monolayer at the air-water interface observed by vibrational sum-frequency generation spectroscopy

Izabela Irena Rzeznicka, Maria Sovago, Ellen H G Backus, Mischa Bonn, Taro Yamada, Toshihide Kobayashi, Maki Kawai

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Duramycin is a small tetracyclic peptide which binds specifically to ethanolamine phospholipids (PE). In this study, we used lipid monolayers consisting of 1-palmitoyl-2-oleoyl phosphatidylethanolamine (POPE) and various phosphatidylcholines (PC) to investigate the effect of duramycin on the organization of lipids and its influence on surrounding water molecules, using vibrational sum-frequency generation spectroscopy in conjunction with surface pressure measurements and fluorescence microscopy. The results show that while duramycin has no effect on the PC lipid monolayers, it induces significant disorder of PE molecules and causes an increase of the PE monolayer surface pressure. Duramycin adopts a β-sheet conformation and is well-ordered at the air-water interface as well as after binding to PE. Our results are consistent with duramycin inserting into the PE monolayer via its hydrophobic end, exposing phenylalanine residues to the lipid. Binding of duramycin to PE broadens the hydrogen-bond distribution of lipid-bound water molecules, notably increasing the fraction of the less strongly hydrogen-bonded, possibly undercoordinated, water molecules. Fluorescence microscopy reveals that the interaction of duramycin with PE causes a change in the shape of the liquid-condensed domains of the PE monolayer from circular to horseshoe-like, indicating a reduction of line tension at the boundary of the two lipid phases. These results reveal that the first steps in the disruption of membrane integrity by duramycin consist of a reduction of the line tension, a decrease in the lipid order, and a weakening of the hydrogen bonding network of water around PE.

Original languageEnglish
Pages (from-to)16055-16062
Number of pages8
JournalLangmuir
Volume26
Issue number20
DOIs
Publication statusPublished - 2010 Oct 19
Externally publishedYes

Fingerprint

destabilization
Vibrational spectra
Lipids
lipids
Monolayers
Spectroscopy
Water
air
Air
water
spectroscopy
Molecules
Fluorescence microscopy
Phosphatidylcholines
molecules
Hydrogen bonds
microscopy
Ethanolamines
fluorescence
phenylalanine

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Duramycin-induced destabilization of a phosphatidylethanolamine monolayer at the air-water interface observed by vibrational sum-frequency generation spectroscopy. / Rzeznicka, Izabela Irena; Sovago, Maria; Backus, Ellen H G; Bonn, Mischa; Yamada, Taro; Kobayashi, Toshihide; Kawai, Maki.

In: Langmuir, Vol. 26, No. 20, 19.10.2010, p. 16055-16062.

Research output: Contribution to journalArticle

Rzeznicka, Izabela Irena ; Sovago, Maria ; Backus, Ellen H G ; Bonn, Mischa ; Yamada, Taro ; Kobayashi, Toshihide ; Kawai, Maki. / Duramycin-induced destabilization of a phosphatidylethanolamine monolayer at the air-water interface observed by vibrational sum-frequency generation spectroscopy. In: Langmuir. 2010 ; Vol. 26, No. 20. pp. 16055-16062.
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