Abstract
Dynamic deformation and recovery responses of red blood cells (RBCs) to a cyclically reversing shear flow generated in a 30-μm clearance, with the peak shear stress of 53, 108, 161, and 274 Pa at the frequency of 1, 2, 3, and 5 Hz, respectively, were studied. The RBCs' time-varying velocity varied after the glass plate velocity without any time lag, whereas the L/W change, where L and W were the major and minor axes of RBCs' ellipsoidal shape, exhibited a rapid increase and gradual decay during the deformation and recovery phase. The time of minimum L/W occurrence lagged behind the zero-velocity time of the glass plate (zero stress), and the delay time normalized to the one-cycle duration remained constant at 8.0%. The elongation of RBCs at zero stress time became larger with the reversing frequency. A simple mechanical model consisting of an elastic linear element during a rapid elongation period and a parallel combination of elements such as a spring and dashpot during the nonlinear recovery phase was suggested. The dynamic response behavior of RBCs under a cyclically reversing shear flow was different from the conventional shape change where a steplike force was applied to and completely released from the RBCs.
| Original language | English |
|---|---|
| Pages (from-to) | 1984-1998 |
| Number of pages | 15 |
| Journal | Biophysical Journal |
| Volume | 91 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
Cite this
Dynamic deformation and recovery response of red blood cells to a cyclically reversing shear flow : Effects of frequency of cyclically reversing shear flow and shear stress level. / Watanabe, Nobuo; Kataoka, Hiroyuki; Yasuda, Toshitaka; Takatani, Setsuo.
In: Biophysical Journal, Vol. 91, No. 5, 2006, p. 1984-1998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic deformation and recovery response of red blood cells to a cyclically reversing shear flow
T2 - Effects of frequency of cyclically reversing shear flow and shear stress level
AU - Watanabe, Nobuo
AU - Kataoka, Hiroyuki
AU - Yasuda, Toshitaka
AU - Takatani, Setsuo
PY - 2006
Y1 - 2006
N2 - Dynamic deformation and recovery responses of red blood cells (RBCs) to a cyclically reversing shear flow generated in a 30-μm clearance, with the peak shear stress of 53, 108, 161, and 274 Pa at the frequency of 1, 2, 3, and 5 Hz, respectively, were studied. The RBCs' time-varying velocity varied after the glass plate velocity without any time lag, whereas the L/W change, where L and W were the major and minor axes of RBCs' ellipsoidal shape, exhibited a rapid increase and gradual decay during the deformation and recovery phase. The time of minimum L/W occurrence lagged behind the zero-velocity time of the glass plate (zero stress), and the delay time normalized to the one-cycle duration remained constant at 8.0%. The elongation of RBCs at zero stress time became larger with the reversing frequency. A simple mechanical model consisting of an elastic linear element during a rapid elongation period and a parallel combination of elements such as a spring and dashpot during the nonlinear recovery phase was suggested. The dynamic response behavior of RBCs under a cyclically reversing shear flow was different from the conventional shape change where a steplike force was applied to and completely released from the RBCs.
AB - Dynamic deformation and recovery responses of red blood cells (RBCs) to a cyclically reversing shear flow generated in a 30-μm clearance, with the peak shear stress of 53, 108, 161, and 274 Pa at the frequency of 1, 2, 3, and 5 Hz, respectively, were studied. The RBCs' time-varying velocity varied after the glass plate velocity without any time lag, whereas the L/W change, where L and W were the major and minor axes of RBCs' ellipsoidal shape, exhibited a rapid increase and gradual decay during the deformation and recovery phase. The time of minimum L/W occurrence lagged behind the zero-velocity time of the glass plate (zero stress), and the delay time normalized to the one-cycle duration remained constant at 8.0%. The elongation of RBCs at zero stress time became larger with the reversing frequency. A simple mechanical model consisting of an elastic linear element during a rapid elongation period and a parallel combination of elements such as a spring and dashpot during the nonlinear recovery phase was suggested. The dynamic response behavior of RBCs under a cyclically reversing shear flow was different from the conventional shape change where a steplike force was applied to and completely released from the RBCs.
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U2 - 10.1529/biophysj.105.060236
DO - 10.1529/biophysj.105.060236
M3 - Article
VL - 91
SP - 1984
EP - 1998
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 5
ER -