Dynamic deformation and recovery response of red blood cells to a cyclically reversing shear flow

Effects of frequency of cyclically reversing shear flow and shear stress level

Nobuo Watanabe, Hiroyuki Kataoka, Toshitaka Yasuda, Setsuo Takatani

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Dynamic deformation and recovery responses of red blood cells (RBCs) to a cyclically reversing shear flow generated in a 30-μm clearance, with the peak shear stress of 53, 108, 161, and 274 Pa at the frequency of 1, 2, 3, and 5 Hz, respectively, were studied. The RBCs' time-varying velocity varied after the glass plate velocity without any time lag, whereas the L/W change, where L and W were the major and minor axes of RBCs' ellipsoidal shape, exhibited a rapid increase and gradual decay during the deformation and recovery phase. The time of minimum L/W occurrence lagged behind the zero-velocity time of the glass plate (zero stress), and the delay time normalized to the one-cycle duration remained constant at 8.0%. The elongation of RBCs at zero stress time became larger with the reversing frequency. A simple mechanical model consisting of an elastic linear element during a rapid elongation period and a parallel combination of elements such as a spring and dashpot during the nonlinear recovery phase was suggested. The dynamic response behavior of RBCs under a cyclically reversing shear flow was different from the conventional shape change where a steplike force was applied to and completely released from the RBCs.

Original languageEnglish
Pages (from-to)1984-1998
Number of pages15
JournalBiophysical Journal
Volume91
Issue number5
DOIs
Publication statusPublished - 2006
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

Cite this

Dynamic deformation and recovery response of red blood cells to a cyclically reversing shear flow : Effects of frequency of cyclically reversing shear flow and shear stress level. / Watanabe, Nobuo; Kataoka, Hiroyuki; Yasuda, Toshitaka; Takatani, Setsuo.

In: Biophysical Journal, Vol. 91, No. 5, 2006, p. 1984-1998.

Research output: Contribution to journalArticle

@article{570feb5b174441f78924fa93a9a413de,
title = "Dynamic deformation and recovery response of red blood cells to a cyclically reversing shear flow: Effects of frequency of cyclically reversing shear flow and shear stress level",
abstract = "Dynamic deformation and recovery responses of red blood cells (RBCs) to a cyclically reversing shear flow generated in a 30-μm clearance, with the peak shear stress of 53, 108, 161, and 274 Pa at the frequency of 1, 2, 3, and 5 Hz, respectively, were studied. The RBCs' time-varying velocity varied after the glass plate velocity without any time lag, whereas the L/W change, where L and W were the major and minor axes of RBCs' ellipsoidal shape, exhibited a rapid increase and gradual decay during the deformation and recovery phase. The time of minimum L/W occurrence lagged behind the zero-velocity time of the glass plate (zero stress), and the delay time normalized to the one-cycle duration remained constant at 8.0{\%}. The elongation of RBCs at zero stress time became larger with the reversing frequency. A simple mechanical model consisting of an elastic linear element during a rapid elongation period and a parallel combination of elements such as a spring and dashpot during the nonlinear recovery phase was suggested. The dynamic response behavior of RBCs under a cyclically reversing shear flow was different from the conventional shape change where a steplike force was applied to and completely released from the RBCs.",
author = "Nobuo Watanabe and Hiroyuki Kataoka and Toshitaka Yasuda and Setsuo Takatani",
year = "2006",
doi = "10.1529/biophysj.105.060236",
language = "English",
volume = "91",
pages = "1984--1998",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "5",

}

TY - JOUR

T1 - Dynamic deformation and recovery response of red blood cells to a cyclically reversing shear flow

T2 - Effects of frequency of cyclically reversing shear flow and shear stress level

AU - Watanabe, Nobuo

AU - Kataoka, Hiroyuki

AU - Yasuda, Toshitaka

AU - Takatani, Setsuo

PY - 2006

Y1 - 2006

N2 - Dynamic deformation and recovery responses of red blood cells (RBCs) to a cyclically reversing shear flow generated in a 30-μm clearance, with the peak shear stress of 53, 108, 161, and 274 Pa at the frequency of 1, 2, 3, and 5 Hz, respectively, were studied. The RBCs' time-varying velocity varied after the glass plate velocity without any time lag, whereas the L/W change, where L and W were the major and minor axes of RBCs' ellipsoidal shape, exhibited a rapid increase and gradual decay during the deformation and recovery phase. The time of minimum L/W occurrence lagged behind the zero-velocity time of the glass plate (zero stress), and the delay time normalized to the one-cycle duration remained constant at 8.0%. The elongation of RBCs at zero stress time became larger with the reversing frequency. A simple mechanical model consisting of an elastic linear element during a rapid elongation period and a parallel combination of elements such as a spring and dashpot during the nonlinear recovery phase was suggested. The dynamic response behavior of RBCs under a cyclically reversing shear flow was different from the conventional shape change where a steplike force was applied to and completely released from the RBCs.

AB - Dynamic deformation and recovery responses of red blood cells (RBCs) to a cyclically reversing shear flow generated in a 30-μm clearance, with the peak shear stress of 53, 108, 161, and 274 Pa at the frequency of 1, 2, 3, and 5 Hz, respectively, were studied. The RBCs' time-varying velocity varied after the glass plate velocity without any time lag, whereas the L/W change, where L and W were the major and minor axes of RBCs' ellipsoidal shape, exhibited a rapid increase and gradual decay during the deformation and recovery phase. The time of minimum L/W occurrence lagged behind the zero-velocity time of the glass plate (zero stress), and the delay time normalized to the one-cycle duration remained constant at 8.0%. The elongation of RBCs at zero stress time became larger with the reversing frequency. A simple mechanical model consisting of an elastic linear element during a rapid elongation period and a parallel combination of elements such as a spring and dashpot during the nonlinear recovery phase was suggested. The dynamic response behavior of RBCs under a cyclically reversing shear flow was different from the conventional shape change where a steplike force was applied to and completely released from the RBCs.

UR - http://www.scopus.com/inward/record.url?scp=33748446963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748446963&partnerID=8YFLogxK

U2 - 10.1529/biophysj.105.060236

DO - 10.1529/biophysj.105.060236

M3 - Article

VL - 91

SP - 1984

EP - 1998

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 5

ER -