Dynamic deformation capability of a red blood cell under a cyclically reciprocating shear stress

N. Watanabe, T. Yasuda, H. Kataoka, S. Takatani

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Red blood cells (RBCs) in the cardiovascular devices are exposed to varying degree of the shear stress from all the directions. However the RBCs' deformability or the deformation capability under such a shear stress is not well understood. In this study, we designed and built a system that can induce a cyclically reciprocating shear stress to a RBC suspension. The arm of the cyclically reciprocating shear stress device was attached to the upper piece of the parallel glass plates between which a suspension of human RBCs (1% hematocrit whole blood diluted in a 32 weight% dextran phosphate buffer solution) was contained. The cyclic reciprocating motion of the upper glass plate of 3.0 mm stroke length was produced using a slider-crank shaft mechanism that was linked to an eccentric cam-motor system. Each rotation of the motor produced a 3.0 mm stroke each in the forward and backward direction of the slider block. The clearance between the two glass plates was adjusted to 30 micrometer. The cyclic reciprocating glass plate apparatus was attached to a light microscope stage (IX71 Olympus with x40 objective lens) for illumination with a 350 watt metal halide light source. A high speed camera (MEMREMCAM fx-K3 Nac, 5000 frames per second with shutter kept open) was attached to the microscope to capture the deformation process of the RBCs under cyclic shear stress. The preliminary result indicated that the correlation between the amplitude of the maximum shear stress and the RBCs' deformability. This indicates a potential application of the cyclic reciprocating device to evaluate the temporal response of the RBCs deformability prior to its destruction. The future study will focus on the study of the relative velocity of the erythrocytes with respect to the velocity of the reciprocating plate.

Original languageEnglish
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages5069-5072
Number of pages4
Volume26 VII
Publication statusPublished - 2004
Externally publishedYes
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: 2004 Sep 12004 Sep 5

Other

OtherConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004
CountryUnited States
CitySan Francisco, CA
Period04/9/104/9/5

Fingerprint

Shear stress
Blood
Cells
Formability
Glass
Suspensions
Microscopes
Metal halides
Dextran
High speed cameras
Cams
Light sources
Lenses
Buffers
Phosphates
Lighting

Keywords

  • Cardiovascular device
  • Cyclically reciprocating shear stress
  • Dynamic deformation capability
  • Red blood cell

ASJC Scopus subject areas

  • Bioengineering

Cite this

Watanabe, N., Yasuda, T., Kataoka, H., & Takatani, S. (2004). Dynamic deformation capability of a red blood cell under a cyclically reciprocating shear stress. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 26 VII, pp. 5069-5072)

Dynamic deformation capability of a red blood cell under a cyclically reciprocating shear stress. / Watanabe, N.; Yasuda, T.; Kataoka, H.; Takatani, S.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 VII 2004. p. 5069-5072.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Watanabe, N, Yasuda, T, Kataoka, H & Takatani, S 2004, Dynamic deformation capability of a red blood cell under a cyclically reciprocating shear stress. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 26 VII, pp. 5069-5072, Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004, San Francisco, CA, United States, 04/9/1.
Watanabe N, Yasuda T, Kataoka H, Takatani S. Dynamic deformation capability of a red blood cell under a cyclically reciprocating shear stress. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 VII. 2004. p. 5069-5072
Watanabe, N. ; Yasuda, T. ; Kataoka, H. ; Takatani, S. / Dynamic deformation capability of a red blood cell under a cyclically reciprocating shear stress. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 VII 2004. pp. 5069-5072
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