Early lethality of β-1,4-galactosyltransferase V-mutant mice by growth retardation

Tadahiro Kumagai, Minoru Tanaka, Minesuke Yokoyama, Takeshi Sato, Tadashi Shinkai, Kiyoshi Furukawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The β-1,4-galactosyltransferase (β-1,4-GalT) V whose human and mouse genes were cloned by us has been suggested to be involved in the biosynthesis of N-glycans and O-glycans, and lactosylceramide. To determine its biological function, β-1,4-GalT V (B4galt5) mutant mice obtained by a gene trap method were analyzed. Analysis of pre- and post-implantation embryos revealed that the B4galt5-/- mice die by E10.5 while B4galt5+/- mice were born and grown normally. Histological study showed that most tissues are formed in B4galt5-/- embryos but their appearance at E10.5 is close to that of B4galt5+/- embryos at E9.0-9.5. The results indicate that the growth is delayed by one to one and half day in B4galt5-/- embryos when compared to B4galt5+/- embryos, which results in early death of the embryos by E10.5, probably due to hematopoietic and/or placental defects.

Original languageEnglish
Pages (from-to)456-459
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume379
Issue number2
DOIs
Publication statusPublished - 2009 Feb 6

Keywords

  • Embryonic lethality
  • Growth retardation
  • Knockout mice
  • Lactosylceramide
  • N-glycans
  • O-glycans
  • β-1,4-Galactosyltransferase V

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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