Early lethality of β-1,4-galactosyltransferase V-mutant mice by growth retardation

Tadahiro Kumagai; Minoru Tanaka; Minesuke Yokoyama; Takeshi Sato; Tadashi Shinkai; Kiyoshi Furukawa

doi:10.1016/j.bbrc.2008.12.078

Early lethality of β-1,4-galactosyltransferase V-mutant mice by growth retardation

Tadahiro Kumagai, Minoru Tanaka, Minesuke Yokoyama, Takeshi Sato, Tadashi Shinkai, Kiyoshi Furukawa

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The β-1,4-galactosyltransferase (β-1,4-GalT) V whose human and mouse genes were cloned by us has been suggested to be involved in the biosynthesis of N-glycans and O-glycans, and lactosylceramide. To determine its biological function, β-1,4-GalT V (B4galt5) mutant mice obtained by a gene trap method were analyzed. Analysis of pre- and post-implantation embryos revealed that the B4galt5^-/- mice die by E10.5 while B4galt5^+/- mice were born and grown normally. Histological study showed that most tissues are formed in B4galt5^-/- embryos but their appearance at E10.5 is close to that of B4galt5^+/- embryos at E9.0-9.5. The results indicate that the growth is delayed by one to one and half day in B4galt5^-/- embryos when compared to B4galt5^+/- embryos, which results in early death of the embryos by E10.5, probably due to hematopoietic and/or placental defects.

Original language	English
Pages (from-to)	456-459
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	379
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2008.12.078
Publication status	Published - 2009 Feb 6
Externally published	Yes

Keywords

Embryonic lethality
Growth retardation
Knockout mice
Lactosylceramide
N-glycans
O-glycans
β-1,4-Galactosyltransferase V

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.12.078

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title = "Early lethality of β-1,4-galactosyltransferase V-mutant mice by growth retardation",

abstract = "The β-1,4-galactosyltransferase (β-1,4-GalT) V whose human and mouse genes were cloned by us has been suggested to be involved in the biosynthesis of N-glycans and O-glycans, and lactosylceramide. To determine its biological function, β-1,4-GalT V (B4galt5) mutant mice obtained by a gene trap method were analyzed. Analysis of pre- and post-implantation embryos revealed that the B4galt5-/- mice die by E10.5 while B4galt5+/- mice were born and grown normally. Histological study showed that most tissues are formed in B4galt5-/- embryos but their appearance at E10.5 is close to that of B4galt5+/- embryos at E9.0-9.5. The results indicate that the growth is delayed by one to one and half day in B4galt5-/- embryos when compared to B4galt5+/- embryos, which results in early death of the embryos by E10.5, probably due to hematopoietic and/or placental defects.",

keywords = "Embryonic lethality, Growth retardation, Knockout mice, Lactosylceramide, N-glycans, O-glycans, β-1,4-Galactosyltransferase V",

author = "Tadahiro Kumagai and Minoru Tanaka and Minesuke Yokoyama and Takeshi Sato and Tadashi Shinkai and Kiyoshi Furukawa",

note = "Funding Information: The paper is dedicated to the late professor Chikashi Tachi at the Tokyo University who encouraged us for many years in the field of reproduction biology. This work was supported by Grants-in-Aid for Scientific Research from Ministry of Education, Science, Culture and Sports of Japan (109240104 and 12680708) and by Research Promotion Fund from Japan Science and Technology Agency.",

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doi = "10.1016/j.bbrc.2008.12.078",

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T1 - Early lethality of β-1,4-galactosyltransferase V-mutant mice by growth retardation

AU - Kumagai, Tadahiro

AU - Tanaka, Minoru

AU - Yokoyama, Minesuke

AU - Sato, Takeshi

AU - Shinkai, Tadashi

AU - Furukawa, Kiyoshi

N1 - Funding Information: The paper is dedicated to the late professor Chikashi Tachi at the Tokyo University who encouraged us for many years in the field of reproduction biology. This work was supported by Grants-in-Aid for Scientific Research from Ministry of Education, Science, Culture and Sports of Japan (109240104 and 12680708) and by Research Promotion Fund from Japan Science and Technology Agency.

PY - 2009/2/6

Y1 - 2009/2/6

N2 - The β-1,4-galactosyltransferase (β-1,4-GalT) V whose human and mouse genes were cloned by us has been suggested to be involved in the biosynthesis of N-glycans and O-glycans, and lactosylceramide. To determine its biological function, β-1,4-GalT V (B4galt5) mutant mice obtained by a gene trap method were analyzed. Analysis of pre- and post-implantation embryos revealed that the B4galt5-/- mice die by E10.5 while B4galt5+/- mice were born and grown normally. Histological study showed that most tissues are formed in B4galt5-/- embryos but their appearance at E10.5 is close to that of B4galt5+/- embryos at E9.0-9.5. The results indicate that the growth is delayed by one to one and half day in B4galt5-/- embryos when compared to B4galt5+/- embryos, which results in early death of the embryos by E10.5, probably due to hematopoietic and/or placental defects.

AB - The β-1,4-galactosyltransferase (β-1,4-GalT) V whose human and mouse genes were cloned by us has been suggested to be involved in the biosynthesis of N-glycans and O-glycans, and lactosylceramide. To determine its biological function, β-1,4-GalT V (B4galt5) mutant mice obtained by a gene trap method were analyzed. Analysis of pre- and post-implantation embryos revealed that the B4galt5-/- mice die by E10.5 while B4galt5+/- mice were born and grown normally. Histological study showed that most tissues are formed in B4galt5-/- embryos but their appearance at E10.5 is close to that of B4galt5+/- embryos at E9.0-9.5. The results indicate that the growth is delayed by one to one and half day in B4galt5-/- embryos when compared to B4galt5+/- embryos, which results in early death of the embryos by E10.5, probably due to hematopoietic and/or placental defects.

KW - Embryonic lethality

KW - Growth retardation

KW - Knockout mice

KW - Lactosylceramide

KW - N-glycans

KW - O-glycans

KW - β-1,4-Galactosyltransferase V

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Early lethality of β-1,4-galactosyltransferase V-mutant mice by growth retardation

Abstract

Keywords

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