Effects of expression of hemA and hemB genes on production of porphyrin in Propionibacterium freudenreichii

Yongzhe Piao, Pornpimon Kiatpapan, Mitsuo Yamashita, Yoshikatsu Murooka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The genus Propionibacterium has a wide range of probiotic activities that are exploited in dairy and fermentation systems such as cheeses, propionic acid, and tetrapyrrole compounds. In order to improve production of tetrapyrrole compounds, we expressed the hemA gene, which encodes δ-aminolevulinic acid (ALA) synthase from Rhodobacter sphaeroides, and the hemB gene, which encodes porphobilinogen (PBG) synthase from Propionibacterium freudenreichii subsp. shermanii IFO12424, either monocistronically or polycistronically in strain IFO12426. The recombinant strains accumulated larger amounts of ALA and PBG, with resultant 28- to 33-fold-higher production of porphyrinogens, such as uroporphyrinogen and coproporphyrinogen, than those observed in strain IFO12426, which harbored the shuttle vector pPK705.

Original languageEnglish
Pages (from-to)7561-7566
Number of pages6
JournalApplied and Environmental Microbiology
Volume70
Issue number12
DOIs
Publication statusPublished - 2004 Dec
Externally publishedYes

Fingerprint

Propionibacterium freudenreichii
porphyrin
porphyrins
aminolevulinic acid
gene
acid
Propionibacterium freudenreichii subsp. shermanii
porphobilinogen
porphobilinogen synthase
Rhodobacter sphaeroides
Propionibacterium
genetic vectors
probiotics
genes
propionic acid
fermentation
cheeses
dairies
fold
effect

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Effects of expression of hemA and hemB genes on production of porphyrin in Propionibacterium freudenreichii. / Piao, Yongzhe; Kiatpapan, Pornpimon; Yamashita, Mitsuo; Murooka, Yoshikatsu.

In: Applied and Environmental Microbiology, Vol. 70, No. 12, 12.2004, p. 7561-7566.

Research output: Contribution to journalArticle

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