Effects of microplastics on lentil (Lens culinaris) seed germination and seedling growth

Y. Sanath K. De Silva, Uma Maheswari Rajagopalan, Hirofumi Kadono, Danyang Li

Research output: Contribution to journalArticlepeer-review

Abstract

Widespread use of plastics and mishandling has resulted in severe environmental issues affecting seed germination and seedling growth. This study investigates the effect of polyethylene microplastics (740–4990 nm PEMPs) on lentil (Lens culinaris) seed germination and seedling growth using Biospeckle Optical Coherence Tomography (bOCT), a technique that we successfully demonstrated earlier in visualizing the internal activity of plants. Lentil seeds were exposed to PEMPs bioassay for seven days with 10, 50, and 100 mg L−1 concentrations. The average speckle contrast was calculated after 0 h, 6 h, 12 h, and 24 h of exposure, and statistically significant differences were observed just after 6 h of exposure under all the treatments. However, with conventional parameters, germination viability, germination rate, root and shoot lengths, fresh and dry seedling weights, and antioxidative enzymes, no significant effect was observed until 2 d of exposure. The results revealed that the presence of PEMPs significantly reduced the internal activity at the initial stages that could be visualized only by the use of bOCT, which has never been observed till now. Our results demonstrated for the first time the effect that microplastics indeed could hinder the internal activity during germination of the seeds, possibly resulting from the physical blockage of pores leading to stunted growth at later stages.

Original languageEnglish
Article number135162
JournalChemosphere
Volume303
DOIs
Publication statusPublished - 2022 Sep

Keywords

  • Biospeckle
  • Microplastics
  • Optical coherence tomography (OCT)
  • Seed germination
  • Seedling growth

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Fingerprint

Dive into the research topics of 'Effects of microplastics on lentil (Lens culinaris) seed germination and seedling growth'. Together they form a unique fingerprint.

Cite this