Electric knife for cell surgery: Local ablation by micro-plasma discharge

Y. Yamanishi, H. Kuriki, S. Sakuma, K. Onda, T. Kawahara, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have firstly succeeded in enucleation of oocyte by using micro-electric knife. The discharged output power and conductive area were controlled to adapt the cellular-order ablation under water and under atmospheric pressure environment. The local area ablation was obtained by the glass shell insulation around the copper wire with silver paste, and which prevent the bubble generation and protein contamination when it was discharged under the water. The width of the ablation region was successfully reduced to more than a half by using the glass shell insulation compare to the conventional electrode. Moreover, the nucleus dyed by Hoechst was also removed successfully with limited damage region which is the difficult task for manual operation with glass capillary. This low cost micro-electric knife has a possibility to extend to fabricate any objective material under various environments and contribute to a new top-down fabrication method in the micro-nano bioengineering field.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages945-948
Number of pages4
DOIs
Publication statusPublished - 2012
Externally publishedYes
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris
Duration: 2012 Jan 292012 Feb 2

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CityParis
Period12/1/2912/2/2

Fingerprint

Ablation
surgery
plasma jets
Surgery
ablation
Plasmas
insulation
Glass
Insulation
glass
cells
bioengineering
gametocytes
Water
Ointments
Silver
water
Atmospheric pressure
Copper
atmospheric pressure

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Yamanishi, Y., Kuriki, H., Sakuma, S., Onda, K., Kawahara, T., & Arai, F. (2012). Electric knife for cell surgery: Local ablation by micro-plasma discharge. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 945-948). [6170342] https://doi.org/10.1109/MEMSYS.2012.6170342

Electric knife for cell surgery : Local ablation by micro-plasma discharge. / Yamanishi, Y.; Kuriki, H.; Sakuma, S.; Onda, K.; Kawahara, T.; Arai, F.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. p. 945-948 6170342.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yamanishi, Y, Kuriki, H, Sakuma, S, Onda, K, Kawahara, T & Arai, F 2012, Electric knife for cell surgery: Local ablation by micro-plasma discharge. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6170342, pp. 945-948, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, 12/1/29. https://doi.org/10.1109/MEMSYS.2012.6170342
Yamanishi Y, Kuriki H, Sakuma S, Onda K, Kawahara T, Arai F. Electric knife for cell surgery: Local ablation by micro-plasma discharge. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. p. 945-948. 6170342 https://doi.org/10.1109/MEMSYS.2012.6170342
Yamanishi, Y. ; Kuriki, H. ; Sakuma, S. ; Onda, K. ; Kawahara, T. ; Arai, F. / Electric knife for cell surgery : Local ablation by micro-plasma discharge. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. pp. 945-948
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