Electrically-induced bubble knife for protein crystallization and processing

Hiroki Kuriki, Sou Takasawa, Shinya Sakuma, Genji Kurisu, Fumihito Arai, Yoko Yamanishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.

Original languageEnglish
Title of host publication2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
PublisherIEEE Computer Society
ISBN (Print)9781479915293
DOIs
Publication statusPublished - 2013
Event2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 - Nagoya
Duration: 2013 Nov 102013 Nov 13

Other

Other2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
CityNagoya
Period13/11/1013/11/13

Fingerprint

Crystallization
Proteins
Processing
Bubbles (in fluids)
Stiction
Crystals
Molecules
Electrolysis
Cavitation
Nucleation
Liquids
Air

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Kuriki, H., Takasawa, S., Sakuma, S., Kurisu, G., Arai, F., & Yamanishi, Y. (2013). Electrically-induced bubble knife for protein crystallization and processing. In 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 [6710474] IEEE Computer Society. https://doi.org/10.1109/MHS.2013.6710474

Electrically-induced bubble knife for protein crystallization and processing. / Kuriki, Hiroki; Takasawa, Sou; Sakuma, Shinya; Kurisu, Genji; Arai, Fumihito; Yamanishi, Yoko.

2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013. IEEE Computer Society, 2013. 6710474.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kuriki, H, Takasawa, S, Sakuma, S, Kurisu, G, Arai, F & Yamanishi, Y 2013, Electrically-induced bubble knife for protein crystallization and processing. in 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013., 6710474, IEEE Computer Society, 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013, Nagoya, 13/11/10. https://doi.org/10.1109/MHS.2013.6710474
Kuriki H, Takasawa S, Sakuma S, Kurisu G, Arai F, Yamanishi Y. Electrically-induced bubble knife for protein crystallization and processing. In 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013. IEEE Computer Society. 2013. 6710474 https://doi.org/10.1109/MHS.2013.6710474
Kuriki, Hiroki ; Takasawa, Sou ; Sakuma, Shinya ; Kurisu, Genji ; Arai, Fumihito ; Yamanishi, Yoko. / Electrically-induced bubble knife for protein crystallization and processing. 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013. IEEE Computer Society, 2013.
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