Electrically-induced bubble knife for protein crystallization and processing

Hiroki Kuriki, Sou Takasawa, Shinya Sakuma, Genji Kurisu, Fumihito Arai, Yoko Yamanishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.

Original languageEnglish
Title of host publication2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
PublisherIEEE Computer Society
ISBN (Print)9781479915293
DOIs
Publication statusPublished - 2013 Jan 1
Event2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 - Nagoya, Japan
Duration: 2013 Nov 102013 Nov 13

Publication series

Name2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013

Conference

Conference2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
CountryJapan
CityNagoya
Period13/11/1013/11/13

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Kuriki, H., Takasawa, S., Sakuma, S., Kurisu, G., Arai, F., & Yamanishi, Y. (2013). Electrically-induced bubble knife for protein crystallization and processing. In 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 [6710474] (2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013). IEEE Computer Society. https://doi.org/10.1109/MHS.2013.6710474