Electrochemical determination of uric acid in urine and serum with uricase/carbon nanotube /carboxymethylcellulose electrode

Toya Fukuda, Hitoshi Muguruma, Hisanori Iwasa, Takeshi Tanaka, Atsunori Hiratsuka, Tetsuo Shimizu, Katsumi Tsuji, Takahide Kishimoto

Research output: Contribution to journalArticle

Abstract

The detection of uric acid in blood and urine is clinically important in terms of suitable diagnosis and self-healthcare. An amperometric thin film biosensor composed of carbon nanotube and uricase enzyme is presented. The CNT is successfully dispersed in aqueous solution with carboxymethylcellulose surfactant. This enables thin film formation by a simple drop-casting layer-by-layer process. The uricase/carboxymethylcellulose dispersed carbon nanotube/gold thin film biosensor shows the best sensing performance compared to that with sodium cholate surfactant in terms of higher current and lower detection potential. The presented procedure shows good performance with neither electron transfer mediator nor complicated process. Cyclic voltammetry exhibited a sensitivity of 233 μA mM−1 cm−2 at +0.35 V, a linear range of 0.02–2.7 mM, and a detection limit of 2.8 μM. We quantify and graph uric acid data in actual physiological samples (serum and urine) for the first time and detection values showed good agreement with those obtained by a conventional analytical method (enzymatic colorimetry kit).

Original languageEnglish
Article number113533
JournalAnalytical Biochemistry
Volume590
DOIs
Publication statusPublished - 2020 Feb 1

Fingerprint

Urate Oxidase
Carboxymethylcellulose Sodium
Carbon Nanotubes
Uric Acid
Biosensors
Surface-Active Agents
Thin films
Electrodes
Sodium Cholate
Colorimetry
Gold
Cyclic voltammetry
Casting
Blood
Electrons
Enzymes

Keywords

  • Biosensor
  • Carboxymethylcellulose
  • Multi-walled carbon nanotube
  • Uric acid
  • Uricase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Electrochemical determination of uric acid in urine and serum with uricase/carbon nanotube /carboxymethylcellulose electrode. / Fukuda, Toya; Muguruma, Hitoshi; Iwasa, Hisanori; Tanaka, Takeshi; Hiratsuka, Atsunori; Shimizu, Tetsuo; Tsuji, Katsumi; Kishimoto, Takahide.

In: Analytical Biochemistry, Vol. 590, 113533, 01.02.2020.

Research output: Contribution to journalArticle

Fukuda, Toya ; Muguruma, Hitoshi ; Iwasa, Hisanori ; Tanaka, Takeshi ; Hiratsuka, Atsunori ; Shimizu, Tetsuo ; Tsuji, Katsumi ; Kishimoto, Takahide. / Electrochemical determination of uric acid in urine and serum with uricase/carbon nanotube /carboxymethylcellulose electrode. In: Analytical Biochemistry. 2020 ; Vol. 590.
@article{f5520a3fe7fe47538872a57ac51ba146,
title = "Electrochemical determination of uric acid in urine and serum with uricase/carbon nanotube /carboxymethylcellulose electrode",
abstract = "The detection of uric acid in blood and urine is clinically important in terms of suitable diagnosis and self-healthcare. An amperometric thin film biosensor composed of carbon nanotube and uricase enzyme is presented. The CNT is successfully dispersed in aqueous solution with carboxymethylcellulose surfactant. This enables thin film formation by a simple drop-casting layer-by-layer process. The uricase/carboxymethylcellulose dispersed carbon nanotube/gold thin film biosensor shows the best sensing performance compared to that with sodium cholate surfactant in terms of higher current and lower detection potential. The presented procedure shows good performance with neither electron transfer mediator nor complicated process. Cyclic voltammetry exhibited a sensitivity of 233 μA mM−1 cm−2 at +0.35 V, a linear range of 0.02–2.7 mM, and a detection limit of 2.8 μM. We quantify and graph uric acid data in actual physiological samples (serum and urine) for the first time and detection values showed good agreement with those obtained by a conventional analytical method (enzymatic colorimetry kit).",
keywords = "Biosensor, Carboxymethylcellulose, Multi-walled carbon nanotube, Uric acid, Uricase",
author = "Toya Fukuda and Hitoshi Muguruma and Hisanori Iwasa and Takeshi Tanaka and Atsunori Hiratsuka and Tetsuo Shimizu and Katsumi Tsuji and Takahide Kishimoto",
year = "2020",
month = "2",
day = "1",
doi = "10.1016/j.ab.2019.113533",
language = "English",
volume = "590",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Electrochemical determination of uric acid in urine and serum with uricase/carbon nanotube /carboxymethylcellulose electrode

AU - Fukuda, Toya

AU - Muguruma, Hitoshi

AU - Iwasa, Hisanori

AU - Tanaka, Takeshi

AU - Hiratsuka, Atsunori

AU - Shimizu, Tetsuo

AU - Tsuji, Katsumi

AU - Kishimoto, Takahide

PY - 2020/2/1

Y1 - 2020/2/1

N2 - The detection of uric acid in blood and urine is clinically important in terms of suitable diagnosis and self-healthcare. An amperometric thin film biosensor composed of carbon nanotube and uricase enzyme is presented. The CNT is successfully dispersed in aqueous solution with carboxymethylcellulose surfactant. This enables thin film formation by a simple drop-casting layer-by-layer process. The uricase/carboxymethylcellulose dispersed carbon nanotube/gold thin film biosensor shows the best sensing performance compared to that with sodium cholate surfactant in terms of higher current and lower detection potential. The presented procedure shows good performance with neither electron transfer mediator nor complicated process. Cyclic voltammetry exhibited a sensitivity of 233 μA mM−1 cm−2 at +0.35 V, a linear range of 0.02–2.7 mM, and a detection limit of 2.8 μM. We quantify and graph uric acid data in actual physiological samples (serum and urine) for the first time and detection values showed good agreement with those obtained by a conventional analytical method (enzymatic colorimetry kit).

AB - The detection of uric acid in blood and urine is clinically important in terms of suitable diagnosis and self-healthcare. An amperometric thin film biosensor composed of carbon nanotube and uricase enzyme is presented. The CNT is successfully dispersed in aqueous solution with carboxymethylcellulose surfactant. This enables thin film formation by a simple drop-casting layer-by-layer process. The uricase/carboxymethylcellulose dispersed carbon nanotube/gold thin film biosensor shows the best sensing performance compared to that with sodium cholate surfactant in terms of higher current and lower detection potential. The presented procedure shows good performance with neither electron transfer mediator nor complicated process. Cyclic voltammetry exhibited a sensitivity of 233 μA mM−1 cm−2 at +0.35 V, a linear range of 0.02–2.7 mM, and a detection limit of 2.8 μM. We quantify and graph uric acid data in actual physiological samples (serum and urine) for the first time and detection values showed good agreement with those obtained by a conventional analytical method (enzymatic colorimetry kit).

KW - Biosensor

KW - Carboxymethylcellulose

KW - Multi-walled carbon nanotube

KW - Uric acid

KW - Uricase

UR - http://www.scopus.com/inward/record.url?scp=85076103105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85076103105&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2019.113533

DO - 10.1016/j.ab.2019.113533

M3 - Article

C2 - 31836385

AN - SCOPUS:85076103105

VL - 590

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

M1 - 113533

ER -