Electrochemical properties of an interface between titanium and fibroblasts L929

Sachiko Hiromoto, Kazuhiko Noda, T. Hanawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Various electrochemical measurements were performed on titanium with and without culturing murine fibroblasts L929 to characterize the effects of cells on interface electrochemical properties between titanium and cells. Open-circuit potential of titanium decreased with L929 cells, which was caused by the shift of equilibrium potential between cathodic and anodic reactions indicated by the decrease in cathodic current density with L929 cells. In cathodic potential step test, the decrease in current density following to the peak current density was delayed with L929 cells, indicating that diffusivity of molecules and ions decreased with the cells. In addition, alternating current impedance measurement and data approximation to the electrical equivalent circuit model revealed that the circuit element for diffusion resistance of biomolecule adsorption layer increased with L929 cells. Consequently, the effect of cells on the interface property is the retardation of diffusion through the biomolecule adsorption layer due to the increase in biomolecule density with extracellular matrix consisting with proteins and glycosaminoglycans generated by the cells.

Original languageEnglish
Pages (from-to)387-396
Number of pages10
JournalElectrochimica Acta
Volume48
Issue number4
DOIs
Publication statusPublished - 2002 Dec 20
Externally publishedYes

Fingerprint

Biomolecules
Fibroblasts
Titanium
Electrochemical properties
Current density
Adsorption
Networks (circuits)
Glycosaminoglycans
Equivalent circuits
Ions
Proteins
Molecules

Keywords

  • Biomaterials
  • Electrochemical interface property
  • Fibroblast cells
  • Titanium

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Analytical Chemistry
  • Electrochemistry

Cite this

Electrochemical properties of an interface between titanium and fibroblasts L929. / Hiromoto, Sachiko; Noda, Kazuhiko; Hanawa, T.

In: Electrochimica Acta, Vol. 48, No. 4, 20.12.2002, p. 387-396.

Research output: Contribution to journalArticle

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AB - Various electrochemical measurements were performed on titanium with and without culturing murine fibroblasts L929 to characterize the effects of cells on interface electrochemical properties between titanium and cells. Open-circuit potential of titanium decreased with L929 cells, which was caused by the shift of equilibrium potential between cathodic and anodic reactions indicated by the decrease in cathodic current density with L929 cells. In cathodic potential step test, the decrease in current density following to the peak current density was delayed with L929 cells, indicating that diffusivity of molecules and ions decreased with the cells. In addition, alternating current impedance measurement and data approximation to the electrical equivalent circuit model revealed that the circuit element for diffusion resistance of biomolecule adsorption layer increased with L929 cells. Consequently, the effect of cells on the interface property is the retardation of diffusion through the biomolecule adsorption layer due to the increase in biomolecule density with extracellular matrix consisting with proteins and glycosaminoglycans generated by the cells.

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