TY - JOUR
T1 - Electroreflectance spectroscopic study of the electron transfer rate of cytochrome c electrostatically immobilized on the ω-carboxyl alkanethiol monolayer modified gold electrode
AU - Feng, Zhi Qiang
AU - Imabayashi, Shinichiro
AU - Kakiuchi, Takashi
AU - Niki, Katsumi
N1 - Funding Information:
We are gratefully indebted to the Ministry of Education, Science and Culture, Japan, for the financial support of Grants-in-Aid for Scientific Research on Priority Area of "Electroorganic Chemistry" (05235219 for K.N.) and Grants-in-Aid for Developmental Scientific Research (0455198 for K.N.). We are also grateful to the NEDO (New Energy and Industrial Technology Development Organization) for the financial support. We thank Dr. T. Sagara (Nagasaki University) for his valuable advice on the ER instrumentation. We also thank Mr. M. Iida for the synthesis of the alkanethiols.
PY - 1995/9/19
Y1 - 1995/9/19
N2 - Horse heart cytochrome c was immobilized electrostatically on self-assembled monolayers (SAMs) of carboxylic-acid-terminated alkanethiols, HS(CH2)nCOOH (n = 2, 10) on gold electrodes, The rate constants of the electrode reaction of cytochrome c at the SAM-modified gold electrodes were determined by the frequency dependence of a.c. modulated UV-visible electroreflectance signals. The apparent standard electrode reaction rate constants of cytochrome c were 880 s-1 and 72 s-1 at the HS(CH2)2COOH and HS(CH2)10COOH SAM modified gold electrodes respectively. The rate constant at HS(CH2)2COOH was much smaller than expected from Marcus theory. However, the rate constant at HS(CH2)10COOH could be explained in terms of the through-bond tunnelling mechanism with tunnelling parameter β = 8.2 nm-1.
AB - Horse heart cytochrome c was immobilized electrostatically on self-assembled monolayers (SAMs) of carboxylic-acid-terminated alkanethiols, HS(CH2)nCOOH (n = 2, 10) on gold electrodes, The rate constants of the electrode reaction of cytochrome c at the SAM-modified gold electrodes were determined by the frequency dependence of a.c. modulated UV-visible electroreflectance signals. The apparent standard electrode reaction rate constants of cytochrome c were 880 s-1 and 72 s-1 at the HS(CH2)2COOH and HS(CH2)10COOH SAM modified gold electrodes respectively. The rate constant at HS(CH2)2COOH was much smaller than expected from Marcus theory. However, the rate constant at HS(CH2)10COOH could be explained in terms of the through-bond tunnelling mechanism with tunnelling parameter β = 8.2 nm-1.
KW - Cytochrome c
KW - Electron transfer rate
KW - Gold electrode
KW - Self-assembled monolayers
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U2 - 10.1016/0022-0728(95)04058-V
DO - 10.1016/0022-0728(95)04058-V
M3 - Article
AN - SCOPUS:0001911878
SN - 0022-0728
VL - 394
SP - 149
EP - 154
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
IS - 1-2
ER -