TY - JOUR
T1 - Enhanced degradation of chitosan by applying plasma treatment in combination with oxidizing agents for potential use as an anticancer agent
AU - Chokradjaroen, Chayanaphat
AU - Rujiravanit, Ratana
AU - Watthanaphanit, Anyarat
AU - Theeramunkong, Sewan
AU - Saito, Nagahiro
AU - Yamashita, Kazuko
AU - Arakawa, Ryuichi
N1 - Funding Information:
CC would like to acknowledge the Thailand Research Fund (TRF) for providing her a Royal Golden Jubilee Ph.D. Scholarship (RGJ-Ph.D.: Grant number PHD/0179/2556). This work was financially supported by the Thailand Research Fund (TRF) under the contract number BRG5480008. The authors thank Surapon Foods Public Co., Ltd. (Thailand) for providing the shrimp shells and the NU-PPC Plasma Chemical Technology Laboratory at Chulalongkorn University (Thailand) for providing the use of solution plasma equipment. All facilities for biological tests were supported by Drug Discovery and Development center at Thammasat University (Thailand).
Publisher Copyright:
© 2017 Elsevier Ltd
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Solution plasma (SP) treatment in combination with oxidizing agents, i.e., hydrogen peroxide (H2O2), potassium persulfate (K2S2O8) and sodium nitrite (NaNO2) were adopted to chitosan degradation in order to achieve fast degradation rate, low chemicals used and high yield of low-molecular-weight chitosan and chitooligosaccharide (COS). Among the studied oxidizing agents, H2O2 was found to be the best choice in terms of appreciable molecular weight reduction without major change in chemical structure of the degraded products of chitosan. By the combination with SP treatment, dilute solution of H2O2 (4–60 mM) was required for effective degradation of chitosan. The combination of SP treatment and dilute solution of H2O2 (60 mM) resulted in the great reduction of molecular weight of chitosan and water-soluble chitosan was obtained as a major product. The resulting water-soluble chitosan was precipitated to obtain COS. An inhibitory effect against cervical cancer cell line (HeLa cells) of COS was also examined.
AB - Solution plasma (SP) treatment in combination with oxidizing agents, i.e., hydrogen peroxide (H2O2), potassium persulfate (K2S2O8) and sodium nitrite (NaNO2) were adopted to chitosan degradation in order to achieve fast degradation rate, low chemicals used and high yield of low-molecular-weight chitosan and chitooligosaccharide (COS). Among the studied oxidizing agents, H2O2 was found to be the best choice in terms of appreciable molecular weight reduction without major change in chemical structure of the degraded products of chitosan. By the combination with SP treatment, dilute solution of H2O2 (4–60 mM) was required for effective degradation of chitosan. The combination of SP treatment and dilute solution of H2O2 (60 mM) resulted in the great reduction of molecular weight of chitosan and water-soluble chitosan was obtained as a major product. The resulting water-soluble chitosan was precipitated to obtain COS. An inhibitory effect against cervical cancer cell line (HeLa cells) of COS was also examined.
KW - Chitooligosaccharide
KW - Chitosan
KW - Degradation
KW - Oxidizing agent
KW - Plasma treatment
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U2 - 10.1016/j.carbpol.2017.03.006
DO - 10.1016/j.carbpol.2017.03.006
M3 - Article
C2 - 28433142
AN - SCOPUS:85015079231
SN - 0144-8617
VL - 167
SP - 1
EP - 11
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -