Enhancement of energy expenditure following a single oral dose of flavan-3-ols associated with an increase in catecholamine secretion

Yusuke Matsumura, Yuta Nakagawa, Katsuyuki Mikome, Hiroki Yamamoto, Naomi Osakabe

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Numerous clinical studies have reported that ingestion of chocolate reduces the risk of metabolic syndrome. However, the mechanisms by which this occurs remain unclear. In this murine study, the metabolic-enhancing activity of a 10 mg/kg mixture of flavan-3-ol fraction derived from cocoa (FL) was compared with the same single dose of (-)-epicatechin (EC). Resting energy expenditure (REE) was significantly increased in mice treated with the FL versus the group administered the distilled water vehicle (Cont) during periods of ad libitum feeding and fasting. Mice were euthanized under the effect of anesthesia 2, 5, and 20 hr after treatment with FL or Cont while subsequently fasting. The mRNA levels of the uncoupling protein-1 (UCP-1) and peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) in brown adipose tissue (BAT) were significantly increased 2 hr after administration of FL. UCP-3 and PGC-1α in the gastrocnemius were significantly increased 2 and 5 hr after administration of the FL. The concentrations of phosphorylated AMP-activated protein kinase (AMPK) 1a were found to be significant in the gastrocnemius of mice 2 and 5 hr after ingesting FL. However, these changes were not observed following treatment with EC. Plasma was collected for measurement of catecholamine levels in other animals euthanized by decapitation 2 and 4 hr after their respective group treatment. Plasma adrenaline level was significantly elevated 2 hr after treatment with FL; however, this change was not observed following the administration of EC alone. The present results indicated that FL significantly enhanced systemic energy expenditure, as evidenced by an accompanying increase in the type of gene expression responsible for thermogenesis and lipolysis, whereas EC exhibited this less robustly or effectively. It was suggested the possible interaction between thermogenic and lipolytic effects and the increase in plasma catecholamine concentrations after administration of a single oral dose of FL.

Original languageEnglish
Article numbere112180
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - 2014 Nov 6

Fingerprint

Catechin
epicatechin
catecholamines
flavanols
energy expenditure
Catecholamines
mouth
secretion
mice
dosage
Plasmas
fasting
Cocoa
AMP-activated protein kinase
resting energy expenditure
decapitation
AMP-Activated Protein Kinases
ad libitum feeding
metabolic studies
brown adipose tissue

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Enhancement of energy expenditure following a single oral dose of flavan-3-ols associated with an increase in catecholamine secretion. / Matsumura, Yusuke; Nakagawa, Yuta; Mikome, Katsuyuki; Yamamoto, Hiroki; Osakabe, Naomi.

In: PLoS One, Vol. 9, No. 11, e112180, 06.11.2014.

Research output: Contribution to journalArticle

Matsumura, Yusuke ; Nakagawa, Yuta ; Mikome, Katsuyuki ; Yamamoto, Hiroki ; Osakabe, Naomi. / Enhancement of energy expenditure following a single oral dose of flavan-3-ols associated with an increase in catecholamine secretion. In: PLoS One. 2014 ; Vol. 9, No. 11.
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