Enterolactone restricts the proliferation of the LNCaP human prostate cancer cell line in vitro

Mark J. McCann, Chris I R Gill, Trevor Linton, D. Berrar, Hugh McGlynn, Ian R. Rowland

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ecological data suggest a long-term diet high in plant material rich in biologically active compounds, such as the lignans, can significantly influence the development of prostate cancer over the lifetime of an individual. The capacity of a pure mammalian lignan, enterolactone (ENL), to influence the proliferation of the LNCaP human prostate cancer cell line was investigated as a function of cell density, metabolic activity, expression and secretion of prostate specific antigen (PSA), cell cycle profile, and the expression of genes involved in development and progression of prostate cancer. Treatment with a subcytotoxic concentration of ENL (60 μM for 72 h) was found to reduce: cell density (57.5%, SD 7.23, p < 0.001), metabolic activity (55%, SD 0.03, p < 0.001), secretion of PSA (48.50% SD 4.74, p = 0.05) and induce apoptosis (8.33-fold SD 0.04, p = 0.001) compared to untreated cells. Cotreatment with 10 μM etoposide was found to increase apoptosis by 50.17% (SD 0.02, p < 0.001). Additionally, several key genes (e. g. MCMs, survivin and CDKs) were beneficially regulated by ENL treatment (p < 0.05). The data suggest that the antiproliferative activity of ENL is a consequence of altered expression of cell cycle associated genes and provides novel molecular evidence for the antiproliferative properties of a pure lignan in prostate cancer.

Original languageEnglish
Pages (from-to)567-580
Number of pages14
JournalMolecular Nutrition and Food Research
Volume52
Issue number5
DOIs
Publication statusPublished - 2008 May
Externally publishedYes

Fingerprint

prostatic neoplasms
lignans
cell lines
prostate-specific antigen
cell cycle
apoptosis
secretion
cells
genes
gene expression
neoplasm cells
diet

Keywords

  • Apoptosis
  • Enterolactone
  • Gene expression
  • LNCaP
  • Proliferation

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Medicine(all)

Cite this

Enterolactone restricts the proliferation of the LNCaP human prostate cancer cell line in vitro. / McCann, Mark J.; Gill, Chris I R; Linton, Trevor; Berrar, D.; McGlynn, Hugh; Rowland, Ian R.

In: Molecular Nutrition and Food Research, Vol. 52, No. 5, 05.2008, p. 567-580.

Research output: Contribution to journalArticle

McCann, Mark J. ; Gill, Chris I R ; Linton, Trevor ; Berrar, D. ; McGlynn, Hugh ; Rowland, Ian R. / Enterolactone restricts the proliferation of the LNCaP human prostate cancer cell line in vitro. In: Molecular Nutrition and Food Research. 2008 ; Vol. 52, No. 5. pp. 567-580.
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