Detoxificación enzimática de cianuro mediante el uso de una dihidratasa parcialmente purificada de la cepa serratia marcescens aq07

Translated title of the contribution: Enzymatic cyanide detoxification by partially purified cyanide dihydratase obtained from serratia marcescens strain aq07

K. I. Karamba, M. K. Sabullah, A. Zulkharnain, C. Gomez-Fuentes, S. A. Ahmad

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The partially purified enzyme of indigenously isolated Serraiia marcescens strain AQ07 was utilised to develop the best form of cyanide detoxification method that is eco-friendry and cost effective. The present study evaluates the feasibility of the enzyme to degrade high cyanide concentrations and the possible metabolic pathways involved, for which the protein concentration and cyanide detoxification activity were quantified. Bacterial cells grown in cyanide incorporated medium were disrupted by sonication and the resultant cell free extract were tested for metabolic pathway. The cell free extract was precipitated by ammonium sulphate precipitation and partially purified by ion exchange chromatography using DKAK cellulose. The maximum enzyme activity achieved was 2125 /Al/min. The partially purified enzyme was found to be able to detoxify 82% of 2 mM KCN in 10 min of incubation and cyanide degradation (or depletion) rate showing a linear increase with increasing enzyme concentration. The effective accruing of ammonia as metabolite illustrated that the detoxification was ensued via the function of cyanide dihydratase. Additional confirmation through SDS-Page showed that the molecular weight of enzyme was assessed to be ~38 kDa. which is tandem with the reported cyanide dihydratases. Hence, the use of enzyme as a substitute to live bacterial cells in detoxification of cyanide illustrates various advantages such as the capacity to withstand and detoxify higher cyanide concentration and total reduction in the total cost of process since nutrient provision is immaterial.

Translated title of the contributionEnzymatic cyanide detoxification by partially purified cyanide dihydratase obtained from serratia marcescens strain aq07
Original languageSpanish
Pages (from-to)717-730
Number of pages14
JournalRevista Mexicana de Ingeniera Qumica
Volume19
Issue number2
DOIs
Publication statusPublished - 2020 May 1

Keywords

  • Cyanide dihydratase
  • Detoxification
  • Senzyme
  • Serratia marcescens

ASJC Scopus subject areas

  • Chemical Engineering(all)

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