Abstract
We report a novel approach to fabrication of an amperometric biosensor with an enzyme, a plasma-polymerized film (PPF), and carbon nanotubes (CNTs). The CNTs were grown directly on an island-patterned Co/Ti/Cr layer on a glass substrate by microwave plasma enhanced chemical vapor deposition. The as-grown CNTs were subsequently treated by nitrogen plasma, which changed the surface from hydrophobic to hydrophilic in order to obtain an electrochemical contact between the CNTs and enzymes. A glucose oxidase (GOx) enzyme was then adsorbed onto the CNT surface and directly treated with acetonitrile plasma to overcoat the GOx layer with a PPF. This fabrication process provides a robust design of CNT-based enzyme biosensor, because of all processes are dry except the procedure for enzyme immobilization. The main novelty of the present methodology lies in the PPF and/or plasma processes. The optimized glucose biosensor revealed a high sensitivity of 38 μA mM-1 cm-2, a broad linear dynamic range of 0.25-19 mM (correlation coefficient of 0.994), selectivity toward an interferent (ascorbic acid), and a fast response time of 7 s. The background current was much smaller in magnitude than the current due to 10 mM glucose response. The low limit of detection was 34 μM (S/N = 3). All results strongly suggest that a plasma-polymerized process can provide a new platform for CNT-based biosensor design.
| Original language | English |
|---|---|
| Pages (from-to) | 2445-2450 |
| Number of pages | 6 |
| Journal | ACS Applied Materials and Interfaces |
| Volume | 3 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2011 Jul 27 |
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Keywords
- acetonitrile monomer
- amperometric biosensor
- carbon nanotube
- direct grown on substrate
- glucose oxidase
- plasma-polymerized film
ASJC Scopus subject areas
- Materials Science(all)
- Medicine(all)
Cite this
Enzyme biosensor based on plasma-polymerized film-covered carbon nanotube layer grown directly on a flat substrate. / Muguruma, Hitoshi; Hoshino, Tatsuya; Matsui, Yasunori.
In: ACS Applied Materials and Interfaces, Vol. 3, No. 7, 27.07.2011, p. 2445-2450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enzyme biosensor based on plasma-polymerized film-covered carbon nanotube layer grown directly on a flat substrate
AU - Muguruma, Hitoshi
AU - Hoshino, Tatsuya
AU - Matsui, Yasunori
PY - 2011/7/27
Y1 - 2011/7/27
N2 - We report a novel approach to fabrication of an amperometric biosensor with an enzyme, a plasma-polymerized film (PPF), and carbon nanotubes (CNTs). The CNTs were grown directly on an island-patterned Co/Ti/Cr layer on a glass substrate by microwave plasma enhanced chemical vapor deposition. The as-grown CNTs were subsequently treated by nitrogen plasma, which changed the surface from hydrophobic to hydrophilic in order to obtain an electrochemical contact between the CNTs and enzymes. A glucose oxidase (GOx) enzyme was then adsorbed onto the CNT surface and directly treated with acetonitrile plasma to overcoat the GOx layer with a PPF. This fabrication process provides a robust design of CNT-based enzyme biosensor, because of all processes are dry except the procedure for enzyme immobilization. The main novelty of the present methodology lies in the PPF and/or plasma processes. The optimized glucose biosensor revealed a high sensitivity of 38 μA mM-1 cm-2, a broad linear dynamic range of 0.25-19 mM (correlation coefficient of 0.994), selectivity toward an interferent (ascorbic acid), and a fast response time of 7 s. The background current was much smaller in magnitude than the current due to 10 mM glucose response. The low limit of detection was 34 μM (S/N = 3). All results strongly suggest that a plasma-polymerized process can provide a new platform for CNT-based biosensor design.
AB - We report a novel approach to fabrication of an amperometric biosensor with an enzyme, a plasma-polymerized film (PPF), and carbon nanotubes (CNTs). The CNTs were grown directly on an island-patterned Co/Ti/Cr layer on a glass substrate by microwave plasma enhanced chemical vapor deposition. The as-grown CNTs were subsequently treated by nitrogen plasma, which changed the surface from hydrophobic to hydrophilic in order to obtain an electrochemical contact between the CNTs and enzymes. A glucose oxidase (GOx) enzyme was then adsorbed onto the CNT surface and directly treated with acetonitrile plasma to overcoat the GOx layer with a PPF. This fabrication process provides a robust design of CNT-based enzyme biosensor, because of all processes are dry except the procedure for enzyme immobilization. The main novelty of the present methodology lies in the PPF and/or plasma processes. The optimized glucose biosensor revealed a high sensitivity of 38 μA mM-1 cm-2, a broad linear dynamic range of 0.25-19 mM (correlation coefficient of 0.994), selectivity toward an interferent (ascorbic acid), and a fast response time of 7 s. The background current was much smaller in magnitude than the current due to 10 mM glucose response. The low limit of detection was 34 μM (S/N = 3). All results strongly suggest that a plasma-polymerized process can provide a new platform for CNT-based biosensor design.
KW - acetonitrile monomer
KW - amperometric biosensor
KW - carbon nanotube
KW - direct grown on substrate
KW - glucose oxidase
KW - plasma-polymerized film
UR - http://www.scopus.com/inward/record.url?scp=80053651382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053651382&partnerID=8YFLogxK
U2 - 10.1021/am200342q
DO - 10.1021/am200342q
M3 - Article
C2 - 21678995
AN - SCOPUS:80053651382
VL - 3
SP - 2445
EP - 2450
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
SN - 1944-8244
IS - 7
ER -