Enzyme biosensor based on plasma-polymerized film-covered carbon nanotube layer grown directly on a flat substrate

Hitoshi Muguruma, Tatsuya Hoshino, Yasunori Matsui

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We report a novel approach to fabrication of an amperometric biosensor with an enzyme, a plasma-polymerized film (PPF), and carbon nanotubes (CNTs). The CNTs were grown directly on an island-patterned Co/Ti/Cr layer on a glass substrate by microwave plasma enhanced chemical vapor deposition. The as-grown CNTs were subsequently treated by nitrogen plasma, which changed the surface from hydrophobic to hydrophilic in order to obtain an electrochemical contact between the CNTs and enzymes. A glucose oxidase (GOx) enzyme was then adsorbed onto the CNT surface and directly treated with acetonitrile plasma to overcoat the GOx layer with a PPF. This fabrication process provides a robust design of CNT-based enzyme biosensor, because of all processes are dry except the procedure for enzyme immobilization. The main novelty of the present methodology lies in the PPF and/or plasma processes. The optimized glucose biosensor revealed a high sensitivity of 38 μA mM-1 cm-2, a broad linear dynamic range of 0.25-19 mM (correlation coefficient of 0.994), selectivity toward an interferent (ascorbic acid), and a fast response time of 7 s. The background current was much smaller in magnitude than the current due to 10 mM glucose response. The low limit of detection was 34 μM (S/N = 3). All results strongly suggest that a plasma-polymerized process can provide a new platform for CNT-based biosensor design.

Original languageEnglish
Pages (from-to)2445-2450
Number of pages6
JournalACS Applied Materials and Interfaces
Volume3
Issue number7
DOIs
Publication statusPublished - 2011 Jul 27

Fingerprint

Carbon Nanotubes
Biosensors
Carbon nanotubes
Enzymes
Plasmas
Substrates
Glucose Oxidase
Glucose oxidase
Glucose
Enzyme immobilization
Nitrogen plasma
Fabrication
Ascorbic acid
Plasma enhanced chemical vapor deposition
Acetonitrile
Ascorbic Acid
Microwaves
Glass

Keywords

  • acetonitrile monomer
  • amperometric biosensor
  • carbon nanotube
  • direct grown on substrate
  • glucose oxidase
  • plasma-polymerized film

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

Enzyme biosensor based on plasma-polymerized film-covered carbon nanotube layer grown directly on a flat substrate. / Muguruma, Hitoshi; Hoshino, Tatsuya; Matsui, Yasunori.

In: ACS Applied Materials and Interfaces, Vol. 3, No. 7, 27.07.2011, p. 2445-2450.

Research output: Contribution to journalArticle

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